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Mouse Anti-EIF2S2 Recombinant Antibody (CBFYE-0667) (V2LY-0425-LY1680)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0667
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
N-terminus of human eIF2β.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 2 Subunit Beta
Entrez Gene ID
Human8894
Mouse67204
Rat296302
UniProt ID
HumanP20042
MouseQ99L45
RatQ6P685
Research Area
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Biological Process
Formation of cytoplasmic translation initiation complex Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
In utero embryonic development Source: Ensembl
Male germ cell proliferation Source: Ensembl
Male gonad development Source: Ensembl
Cellular Location
Cytosol; Cytoplasm; Eukaryotic translation initiation factor 2 complex
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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