Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (V2LY-0425-LY1781)

Name: Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745)
SKU: V2LY-0425-LY1781
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (V2LY-0425-LY1781-1) in WB

Western Blot analysis of eIF4G, eIF4B, eIF4H expression in LN18 cells treated with 2.5 nM of siRNA control (siCtr), siRNAs against hnRNP H and hnRNP F (siH and siF) for 72 h. ...View More

Le Bras, M., Gorelick, N., Pautet, S., Tyler, B., Manenti, S., Skuli, N., ... & Cammas, A. (2022). Translational regulation by hnRNP H/F is essential for the proliferation and survival of glioblastoma. Cancers, 14(5), 1283.

Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (V2LY-0425-LY1781-2) in WB

Western blotting was performed using indicated antibodies (top). The band intensity of siSTAT3 group was normalized to that of siCTRL group in each cell line (bottom; n = 2-6). Long, long exposure; Short, short exposure. ...View More

Lee, H., Chin, H., Kim, H., Jung, H., & Lee, D. (2020). STAT3-mediated MLST8 gene expression regulates cap-dependent translation in cancer cells. Molecular Oncology, 14(8), 1850-1867.

Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (V2LY-0425-LY1781-3) in Immunoblot

Western immunoblots were used to define changes in eIF4B or eIF4H protein levels from 5 independent control or 4 independent C9+ derived fibroblast cell lines. Data are relative to controls. Quantification of total protein was done after normalizing to loading, using GAPDH. Phospho-eIF4B quantification was further normalized to total eIF4B. Statistics: unpaired student t-tests. Shown: each data point represents 1 cell line with mean ± SEM; the mean data from 2 independent protein preparations is shown per line. ...View More

Goodman, L. D., Prudencio, M., Srinivasan, A. R., Rifai, O. M., Lee, V. M. Y., Petrucelli, L., & Bonini, N. M. (2019). eIF4B and eIF4H mediate GR production from expanded G4C2 in a Drosophila model for C9orf72-associated ALS. Acta Neuropathologica Communications, 7, 1-16.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYE-0745

Application

WB, IP, IF

Immunogen

Synthetic peptide corresponding to the sequence of human eIF4H.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IP	1:50
IF(ICC)	1:200-1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & HEPES

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 4H

Entrez Gene ID

Human	7458
Mouse	22384
Rat	288599
Monkey	693429

UniProt ID

Human	Q15056
Mouse	Q9WUK2
Rat	Q5XI72
Monkey	F7HNN2

Research Area

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.

Biological Process

Developmental growth Source: Ensembl
Eukaryotic translation initiation factor 4F complex assembly Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
Regulation of translational initiation Source: ProtInc
Sexual reproduction Source: Ensembl

Cellular Location

Perinuclear region

Involvement in disease

EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

Teo, C. S. H., & O’Hare, P. (2018). A bimodal switch in global protein translation coupled to eIF4H relocalisation during advancing cell-cell transmission of herpes simplex virus. PLoS Pathogens, 14(7), e1007196.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (CAT#: CBMAB-E1178-FY)

Related Products

Mouse Anti-EIF4H Recombinant Antibody (4B2) (CAT#: CBMAB-A9909-LY)

Mouse Anti-EIF4H Recombinant Antibody (CBFYE-0746) (CAT#: CBMAB-E1179-FY)

Rabbit Anti-EIF4H Recombinant Antibody (CBFYE-0745) (CAT#: CBMAB-E1178-FY)

Mouse Anti-EIF4H Recombinant Antibody (CBFYE-0746) (CAT#: V2LY-0425-LY1782)

Mouse Anti-EIF4H Recombinant Antibody (4B2) (CAT#: V2LY-0425-LY1783)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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