Mouse Anti-ENO1 Recombinant Antibody (CBFYE-0084) (CBMAB-E0178-FY)

Name: Mouse Anti-ENO1 Recombinant Antibody (CBFYE-0084)
SKU: CBMAB-E0178-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0084

Application

WB, IP, ELISA

Immunogen

Recombinant human α Enolase.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Enolase 1

Introduction

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Entrez Gene ID

Human	2023
Mouse	13806
Rat	24333

UniProt ID

Human	P06733
Mouse	P17182
Rat	P04764

Alternative Names

Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1

Research Area

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209).

In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581).

May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133).

Stimulates immunoglobulin production (PubMed:1369209).

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Biological Process

Canonical glycolysis Source: UniProtKB
Glycolytic process Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway Source: CAFA
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: CAFA
Positive regulation of ATP biosynthetic process Source: CAFA
Positive regulation of muscle contraction Source: CAFA
Positive regulation of plasminogen activation Source: CAFA
Regulation of vacuole fusion, non-autophagic Source: GO_Central
Response to virus Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm; M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Isoform MBP-1: Nucleus

PTM

ISGylated.
Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Huppertz, I., Perez-Perri, J. I., Mantas, P., Sekaran, T., Schwarzl, T., Russo, F., ... & Hentze, M. W. (2022). Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation. Molecular Cell, 82(14), 2666-2680.

Qiao, G., Wu, A., Chen, X., Tian, Y., & Lin, X. (2021). Enolase 1, a moonlighting protein, as a potential target for cancer treatment. International journal of biological sciences, 17(14), 3981.

Xu, W., Yang, W., Wu, C., Ma, X., Li, H., & Zheng, J. (2021). Enolase 1 correlated with cancer progression and immune-infiltrating in multiple cancer types: a pan-cancer analysis. Frontiers in oncology, 10, 593706.

Yang, T., Shu, X., Zhang, H. W., Sun, L. X., Yu, L., Liu, J., ... & Ran, Y. L. (2020). Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis. Cell Death & Disease, 11(10), 1-13.

Chen, J. M. M., Chiu, S. C., Chen, K. C., Huang, Y. R. J., Liao, Y. T. A., & Yu, C. T. R. (2020). Enolase 1 differentially contributes to cell transformation in lung cancer but not in esophageal cancer. Oncology letters, 19(4), 3189-3196.

Didiasova, M., Schaefer, L., & Wygrecka, M. (2019). When place matters: shuttling of enolase-1 across cellular compartments. Frontiers in cell and developmental biology, 7, 61.

Cancemi, P., Buttacavoli, M., Roz, E., & Feo, S. (2019). Expression of alpha-enolase (ENO1), myc promoter-binding protein-1 (MBP-1) and matrix metalloproteinases (MMP-2 and MMP-9) reflect the nature and aggressiveness of breast tumors. International journal of molecular sciences, 20(16), 3952.

Lee, J. Y., Kang, M. J., Choi, J. Y., Park, J. S., Park, J. K., Lee, E. Y., ... & Song, Y. W. (2018). Apolipoprotein B binds to enolase-1 and aggravates inflammation in rheumatoid arthritis. Annals of the Rheumatic Diseases, 77(10), 1480-1489.

Zhu, W., Li, H., Yu, Y., Chen, J., Chen, X., Ren, F., ... & Cui, G. (2018). Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients. Cancer management and research, 10, 5735.

Liu, Y., Li, H., Liu, Y., & Zhu, Z. (2018). MiR-22-3p targeting alpha-enolase 1 regulates the proliferation of retinoblastoma cells. Biomedicine & Pharmacotherapy, 105, 805-812.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ENO1 Matched Antibody Pair (377) (CAT#: APMAB-377LY)

ENO1 Matched Antibody Pair (378) (CAT#: APMAB-378LY)

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Isotype control

For research use only. Not intended for any clinical use.

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