Sign in or Register   Sign in or Register
  |  

Rabbit Anti-ENO1 Recombinant Antibody (CBFYE-0138) (CBMAB-E0301-FY)

This product is rabbit antibody that recognizes ENO1. The antibody CBFYE-0138 can be used for immunoassay techniques such as: WB, IP, IF.
See all ENO1 antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
CBFYE-0138
Antibody Isotype
IgG
Application
WB, IP, IF

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Enolase 1
Introduction
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Entrez Gene ID
Human2023
Mouse13806
Rat24333
UniProt ID
HumanP06733
MouseP17182
RatP04764
Alternative Names
Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1
Research Area
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209).

In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581).

May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133).

Stimulates immunoglobulin production (PubMed:1369209).

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Biological Process
Canonical glycolysis Source: UniProtKB
Glycolytic process Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway Source: CAFA
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: CAFA
Positive regulation of ATP biosynthetic process Source: CAFA
Positive regulation of muscle contraction Source: CAFA
Positive regulation of plasminogen activation Source: CAFA
Regulation of vacuole fusion, non-autophagic Source: GO_Central
Response to virus Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Isoform MBP-1: Nucleus
PTM
ISGylated.
Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Huppertz, I., Perez-Perri, J. I., Mantas, P., Sekaran, T., Schwarzl, T., Russo, F., ... & Hentze, M. W. (2022). Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation. Molecular Cell, 82(14), 2666-2680.

Qiao, G., Wu, A., Chen, X., Tian, Y., & Lin, X. (2021). Enolase 1, a moonlighting protein, as a potential target for cancer treatment. International journal of biological sciences, 17(14), 3981.

Xu, W., Yang, W., Wu, C., Ma, X., Li, H., & Zheng, J. (2021). Enolase 1 correlated with cancer progression and immune-infiltrating in multiple cancer types: a pan-cancer analysis. Frontiers in oncology, 10, 593706.

Yang, T., Shu, X., Zhang, H. W., Sun, L. X., Yu, L., Liu, J., ... & Ran, Y. L. (2020). Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis. Cell Death & Disease, 11(10), 1-13.

Chen, J. M. M., Chiu, S. C., Chen, K. C., Huang, Y. R. J., Liao, Y. T. A., & Yu, C. T. R. (2020). Enolase 1 differentially contributes to cell transformation in lung cancer but not in esophageal cancer. Oncology letters, 19(4), 3189-3196.

Didiasova, M., Schaefer, L., & Wygrecka, M. (2019). When place matters: shuttling of enolase-1 across cellular compartments. Frontiers in cell and developmental biology, 7, 61.

Cancemi, P., Buttacavoli, M., Roz, E., & Feo, S. (2019). Expression of alpha-enolase (ENO1), myc promoter-binding protein-1 (MBP-1) and matrix metalloproteinases (MMP-2 and MMP-9) reflect the nature and aggressiveness of breast tumors. International journal of molecular sciences, 20(16), 3952.

Lee, J. Y., Kang, M. J., Choi, J. Y., Park, J. S., Park, J. K., Lee, E. Y., ... & Song, Y. W. (2018). Apolipoprotein B binds to enolase-1 and aggravates inflammation in rheumatoid arthritis. Annals of the Rheumatic Diseases, 77(10), 1480-1489.

Zhu, W., Li, H., Yu, Y., Chen, J., Chen, X., Ren, F., ... & Cui, G. (2018). Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients. Cancer management and research, 10, 5735.

Liu, Y., Li, H., Liu, Y., & Zhu, Z. (2018). MiR-22-3p targeting alpha-enolase 1 regulates the proliferation of retinoblastoma cells. Biomedicine & Pharmacotherapy, 105, 805-812.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-ENO1 Recombinant Antibody (CBFYE-0138)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare