Rat Recombinant ENO1 protein, His Tag (V2LY-0526-LY9347)

Name: Rat Recombinant ENO1 protein, His Tag
SKU: V2LY-0526-LY9347
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant ENO1 protein, His Tag consist of Amino Acid: 1-434 and predicts a molecular mass of 49.4 kDa.

Molecule Mass

49.4 kDa

Sequence

Amino Acid: 1-434

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Enolase 1

Research Area

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209).

In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581).

May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133).

Stimulates immunoglobulin production (PubMed:1369209).

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Biological Process

Canonical glycolysis Source: UniProtKB
Glycolytic process Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway Source: CAFA
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: CAFA
Positive regulation of ATP biosynthetic process Source: CAFA
Positive regulation of muscle contraction Source: CAFA
Positive regulation of plasminogen activation Source: CAFA
Regulation of vacuole fusion, non-autophagic Source: GO_Central
Response to virus Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm; M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Isoform MBP-1: Nucleus

PTM

ISGylated.
Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Huppertz, I., Perez-Perri, J. I., Mantas, P., Sekaran, T., Schwarzl, T., Russo, F., ... & Hentze, M. W. (2022). Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation. Molecular Cell, 82(14), 2666-2680.

Qiao, G., Wu, A., Chen, X., Tian, Y., & Lin, X. (2021). Enolase 1, a moonlighting protein, as a potential target for cancer treatment. International journal of biological sciences, 17(14), 3981.

Xu, W., Yang, W., Wu, C., Ma, X., Li, H., & Zheng, J. (2021). Enolase 1 correlated with cancer progression and immune-infiltrating in multiple cancer types: a pan-cancer analysis. Frontiers in oncology, 10, 593706.

Yang, T., Shu, X., Zhang, H. W., Sun, L. X., Yu, L., Liu, J., ... & Ran, Y. L. (2020). Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis. Cell Death & Disease, 11(10), 1-13.

Chen, J. M. M., Chiu, S. C., Chen, K. C., Huang, Y. R. J., Liao, Y. T. A., & Yu, C. T. R. (2020). Enolase 1 differentially contributes to cell transformation in lung cancer but not in esophageal cancer. Oncology letters, 19(4), 3189-3196.

Didiasova, M., Schaefer, L., & Wygrecka, M. (2019). When place matters: shuttling of enolase-1 across cellular compartments. Frontiers in cell and developmental biology, 7, 61.

Cancemi, P., Buttacavoli, M., Roz, E., & Feo, S. (2019). Expression of alpha-enolase (ENO1), myc promoter-binding protein-1 (MBP-1) and matrix metalloproteinases (MMP-2 and MMP-9) reflect the nature and aggressiveness of breast tumors. International journal of molecular sciences, 20(16), 3952.

Lee, J. Y., Kang, M. J., Choi, J. Y., Park, J. S., Park, J. K., Lee, E. Y., ... & Song, Y. W. (2018). Apolipoprotein B binds to enolase-1 and aggravates inflammation in rheumatoid arthritis. Annals of the Rheumatic Diseases, 77(10), 1480-1489.

Zhu, W., Li, H., Yu, Y., Chen, J., Chen, X., Ren, F., ... & Cui, G. (2018). Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients. Cancer management and research, 10, 5735.

Liu, Y., Li, H., Liu, Y., & Zhu, Z. (2018). MiR-22-3p targeting alpha-enolase 1 regulates the proliferation of retinoblastoma cells. Biomedicine & Pharmacotherapy, 105, 805-812.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ENO1 protein, His Tag (CAT#: V2LY-0526-LY3841)

Human Recombinant ENO2 protein, His Tag (CAT#: V2LY-0526-LY3842)

ENO1 Matched Antibody Pair (377) (CAT#: APMAB-377LY)

ENO1 Matched Antibody Pair (378) (CAT#: APMAB-378LY)

Antibody Pairs

ENO1 Matched Antibody Pair (377) (CAT#: APMAB-377LY)

ENO1 Matched Antibody Pair (378) (CAT#: APMAB-378LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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