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Mouse Anti-ENO1 Recombinant Antibody (1G1) (CBMAB-A2610-LY)

The product is antibody recognizes ENO1. The antibody 1G1 immunoassay techniques such as: WB, ELISA.
See all ENO1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
1G1
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Enolase 1
Introduction
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
Entrez Gene ID
Human2023
Mouse13806
UniProt ID
HumanP06733
MouseP17182
Alternative Names
ENO1L1; MBP-1; MPB1; NNE; PPH
Research Area
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209).

In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581).

May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133).

Stimulates immunoglobulin production (PubMed:1369209).

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Biological Process
Canonical glycolysis Source: UniProtKB
Glycolytic process Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway Source: CAFA
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: CAFA
Positive regulation of ATP biosynthetic process Source: CAFA
Positive regulation of muscle contraction Source: CAFA
Positive regulation of plasminogen activation Source: CAFA
Regulation of vacuole fusion, non-autophagic Source: GO_Central
Response to virus Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Isoform MBP-1: Nucleus
PTM
ISGylated.
Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Huppertz, I., Perez-Perri, J. I., Mantas, P., Sekaran, T., Schwarzl, T., Russo, F., ... & Hentze, M. W. (2022). Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation. Molecular Cell, 82(14), 2666-2680.

Qiao, G., Wu, A., Chen, X., Tian, Y., & Lin, X. (2021). Enolase 1, a moonlighting protein, as a potential target for cancer treatment. International journal of biological sciences, 17(14), 3981.

Xu, W., Yang, W., Wu, C., Ma, X., Li, H., & Zheng, J. (2021). Enolase 1 correlated with cancer progression and immune-infiltrating in multiple cancer types: a pan-cancer analysis. Frontiers in oncology, 10, 593706.

Yang, T., Shu, X., Zhang, H. W., Sun, L. X., Yu, L., Liu, J., ... & Ran, Y. L. (2020). Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis. Cell Death & Disease, 11(10), 1-13.

Chen, J. M. M., Chiu, S. C., Chen, K. C., Huang, Y. R. J., Liao, Y. T. A., & Yu, C. T. R. (2020). Enolase 1 differentially contributes to cell transformation in lung cancer but not in esophageal cancer. Oncology letters, 19(4), 3189-3196.

Didiasova, M., Schaefer, L., & Wygrecka, M. (2019). When place matters: shuttling of enolase-1 across cellular compartments. Frontiers in cell and developmental biology, 7, 61.

Cancemi, P., Buttacavoli, M., Roz, E., & Feo, S. (2019). Expression of alpha-enolase (ENO1), myc promoter-binding protein-1 (MBP-1) and matrix metalloproteinases (MMP-2 and MMP-9) reflect the nature and aggressiveness of breast tumors. International journal of molecular sciences, 20(16), 3952.

Lee, J. Y., Kang, M. J., Choi, J. Y., Park, J. S., Park, J. K., Lee, E. Y., ... & Song, Y. W. (2018). Apolipoprotein B binds to enolase-1 and aggravates inflammation in rheumatoid arthritis. Annals of the Rheumatic Diseases, 77(10), 1480-1489.

Zhu, W., Li, H., Yu, Y., Chen, J., Chen, X., Ren, F., ... & Cui, G. (2018). Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients. Cancer management and research, 10, 5735.

Liu, Y., Li, H., Liu, Y., & Zhu, Z. (2018). MiR-22-3p targeting alpha-enolase 1 regulates the proliferation of retinoblastoma cells. Biomedicine & Pharmacotherapy, 105, 805-812.

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For research use only. Not intended for any clinical use.

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