Mouse Anti-ESR2 Recombinant Antibody (7B10) (CBMAB-E1884-FY)

Name: Mouse Anti-ESR2 Recombinant Antibody (7B10)
SKU: CBMAB-E1884-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7B10

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-150 of human ESR2 expressed in E. coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 1-153

More Infomation

Target

Full Name

Estrogen Receptor 2

Introduction

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.

Entrez Gene ID

Human	2100
Mouse	13983
Rat	25149
Pig	396697

UniProt ID

Human	Q92731
Mouse	O08537
Rat	Q62986
Pig	Q9XSW2

Alternative Names

Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2; Oestrogen Receptor Beta; Estrogen Receptor Beta; ER-BETA; ESTRB; NR3A2

Research Area

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).

Isoform 2:
Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Biological Process

Cell-cell signaling Source: ProtInc
Cellular response to estradiol stimulus Source: CAFA
Intracellular estrogen receptor signaling pathway Source: CAFA
Intracellular steroid hormone receptor signaling pathway Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: CAFA
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Nucleus

Involvement in disease

Ovarian dysgenesis 8 (ODG8):
A form of ovarian dysgenesis, a disorder characterized by lack of spontaneous pubertal development, primary amenorrhea, uterine hypoplasia, and hypergonadotropic hypogonadism as a result of streak gonads.

PTM

Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.

Dalal, H., Dahlgren, M., Gladchuk, S., Brueffer, C., Gruvberger-Saal, S. K., & Saal, L. H. (2022). Clinical associations of ESR2 (estrogen receptor beta) expression across thousands of primary breast tumors. Scientific reports, 12(1), 1-12.

He, J., Yan, J. J., Zha, X., Ding, X. J., Zhang, Y. L., Lu, Z., & Xu, X. H. (2022). Sexually dimorphic effects of estrogen receptor 2 deletion in the dorsal raphe nucleus on emotional behaviors. Journal of Neuroendocrinology, e13195.

Asiamah, C. A., Liu, Y., Ye, R., Pan, Y., Lu, L. L., Zou, K., ... & Su, Y. (2022). Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck. Poultry Science, 101(3), 101630.

Gregorio, K. C. R., Laurindo, C. P., & Machado, U. F. (2021). Estrogen and glycemic homeostasis: the fundamental role of nuclear estrogen receptors ESR1/ESR2 in glucose transporter GLUT4 regulation. Cells, 10(1), 99.

Ulhaq, Z. S., & Garcia, C. P. (2021). Estrogen receptor beta (ESR2) gene polymorphism and susceptibility to dementia. Acta Neurologica Belgica, 121(5), 1281-1293.

Chmielewska, M., Janusz, P., Andrusiewicz, M., Kotwicki, T., & Kotwicka, M. (2020). Methylation of estrogen receptor 2 (ESR2) in deep paravertebral muscles and its association with idiopathic scoliosis. Scientific reports, 10(1), 1-14.

Bahia, W., Soltani, I., Haddad, A., Soua, A., Radhouani, A., Mahdhi, A., & Ferchichi, S. (2020). Association of genetic variants in Estrogen receptor (ESR) 1 and ESR2 with susceptibility to recurrent pregnancy loss in Tunisian women: A case control study. Gene, 736, 144406.

Lang-Muritano, M., Sproll, P., Wyss, S., Kolly, A., Hürlimann, R., Konrad, D., & Biason-Lauber, A. (2018). Early-onset complete ovarian failure and lack of puberty in a woman with mutated estrogen receptor β (ESR2). The Journal of Clinical Endocrinology & Metabolism, 103(10), 3748-3756.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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