Rabbit Anti-FUBP1 Recombinant Antibody (
7C3) (V2LY-0725-LY973)

Name: Rabbit Anti-FUBP1 Recombinant Antibody (7C3)
SKU: V2LY-0725-LY973
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in WB

Western Blot
Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate
All lanes: FUBP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68, 69 kDa
Observed band size: 69 kDa

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in IHC

IHC image of V2LY-0725-LY973 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in IHC

IHC image of V2LY-0725-LY973 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY973 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in FC

Overlay histogram showing Jurkat cells stained with V2LY-0725-LY973 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of>10,000 events was performed.

Rabbit Anti-FUBP1 Recombinant Antibody (7C3) (V2LY-0725-LY973) in IP

Immunoprecipitating FUBP1 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY973 in Jurkat whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY973(2µg)+ Jurkat whole cell lysate(500µg)
Lane 3: Jurkat whole cell lysate (10µg)

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7C3

Application

ELISA, WB, IHC, IF, FC, IP

Immunogen

A synthesized peptide derived from human FUBP1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

far upstream element (FUSE) binding protein 1

Entrez Gene ID

8880

UniProt ID

Q96AE4

Function

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

Biological Process

Positive regulation of gene expression Source: UniProtKB
Regulation of gene expression Source: GO_Central

Cellular Location

Nucleus

PTM

Ubiquitinated. This targets the protein for proteasome-mediated degradation.

Yin, H., Gao, T., Xie, J., Huang, Z., Zhang, X., Yang, F., ... & Yang, X. (2021). FUBP1 promotes colorectal cancer stemness and metastasis via DVL1‐mediated activation of Wnt/β‐catenin signaling. Molecular Oncology, 15(12), 3490-3512.

Wang, X., Xing, L., Yang, R., Chen, H., Wang, M., Jiang, R., ... & Chen, J. (2021). The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC. Molecular Cancer, 20(1), 1-21.

Qian, X., Yang, J., Qiu, Q., Li, X., Jiang, C., Li, J., ... & Lu, Y. (2021). LCAT3, a novel m6A-regulated long non-coding RNA, plays an oncogenic role in lung cancer via binding with FUBP1 to activate c-MYC. Journal of hematology & oncology, 14, 1-19.

Han, T., Wu, Y., Hu, X., Chen, Y., Jia, W., He, Q., ... & Wan, X. (2020). NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis. Cell Death & Disease, 11(6), 473.

Kang, M., Kim, H. J., Kim, T. J., Byun, J. S., Lee, J. H., Lee, D. H., ... & Kim, D. Y. (2020). Multiple functions of Fubp1 in cell cycle progression and cell survival. Cells, 9(6), 1347.

Debaize, L., & Troadec, M. B. (2019). The master regulator FUBP1: its emerging role in normal cell function and malignant development. Cellular and Molecular Life Sciences, 76, 259-281.

Hoang, V. T., Verma, D., Godavarthy, P. S., Llavona, P., Steiner, M., Gerlach, K., ... & Krause, D. S. (2019). The transcriptional regulator FUBP1 influences disease outcome in murine and human myeloid leukemia. Leukemia, 33(7), 1700-1712.

Elman, J. S., Ni, T. K., Mengwasser, K. E., Jin, D., Wronski, A., Elledge, S. J., & Kuperwasser, C. (2019). Identification of FUBP1 as a long tail cancer driver and widespread regulator of tumor suppressor and oncogene alternative splicing. Cell reports, 28(13), 3435-3449.

Jiang, P., Huang, M., Qi, W., Wang, F., Yang, T., Gao, T., ... & Yang, X. (2019). FUBP1 promotes neuroblastoma proliferation via enhancing glycolysis-a new possible marker of malignancy for neuroblastoma. Journal of Experimental & Clinical Cancer Research, 38(1), 1-19.

Debaize, L., Jakobczyk, H., Avner, S., Gaudichon, J., Rio, A. G., Sérandour, A. A., ... & Troadec, M. B. (2018). Interplay between transcription regulators RUNX1 and FUBP1 activates an enhancer of the oncogene c-KIT and amplifies cell proliferation. Nucleic Acids Research, 46(21), 11214-11228.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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