Mouse Anti-HAAO Recombinant Antibody (CBFYH-0645) (CBMAB-H0551-FY)

Name: Mouse Anti-HAAO Recombinant Antibody (CBFYH-0645)
SKU: CBMAB-H0551-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0645

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

3-hydroxyanthranilate 3,4-dioxygenase

Introduction

3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Entrez Gene ID

23498

UniProt ID

P46952

Alternative Names

3-Hydroxyanthranilate 3,4-Dioxygenase; 3-Hydroxyanthranilic Acid Dioxygenase; 3-Hydroxyanthranilate Oxygenase; EC 1.13.11.6; 3-HAO; HAD; VCRL1; HAO

Function

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Biological Process

de novo' NAD biosynthetic process from tryptophan Source: GO_Central
Anthranilate metabolic process Source: UniProtKB-UniRule
NAD biosynthetic process Source: UniProtKB
Neuron cellular homeostasis Source: UniProtKB
Quinolinate biosynthetic process Source: UniProtKB
Quinolinate metabolic process Source: GO_Central
Response to cadmium ion Source: UniProtKB
Response to zinc ion Source: UniProtKB
Tryptophan catabolic process Source: UniProtKB-UniRule

Cellular Location

Cytosol

Involvement in disease

Vertebral, cardiac, renal, and limb defects syndrome 1 (VCRL1):
An autosomal recessive congenital malformation syndrome characterized by vertebral segmentation abnormalities, congenital cardiac defects, renal defects, and distal mild limb defects.

Sanz, I., Altomare, A., Mondanelli, G., Protti, M., Valsecchi, V., Mercolini, L., ... & Regazzoni, L. (2022). Chromatographic measurement of 3-hydroxyanthranilate 3, 4-dioxygenase activity reveals that edaravone can mitigate the formation of quinolinic acid through a direct enzyme inhibition. Journal of Pharmaceutical and Biomedical Analysis, 219, 114948.

Wang, Y., Liu, K. F., Yang, Y., Davis, I., & Liu, A. (2020). Observing 3-hydroxyanthranilate-3, 4-dioxygenase in action through a crystalline lens. Proceedings of the National Academy of Sciences, 117(33), 19720-19730.

Yang, Y., Liu, F., & Liu, A. (2018). Adapting to oxygen: 3-Hydroxyanthrinilate 3, 4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities. Journal of Biological Chemistry, 293(27), 10415-10424.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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