Rat Recombinant HAAO protein, His Tag (V2LY-0526-LY9409)

Name: Rat Recombinant HAAO protein, His Tag
SKU: V2LY-0526-LY9409
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant HAAO protein, His Tag consist of Amino Acid: 1-286 and predicts a molecular mass of 34.8 kDa.

Molecule Mass

34.8 kDa

Sequence

Amino Acid: 1-286

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

3-hydroxyanthranilate 3,4-dioxygenase

Function

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Biological Process

de novo' NAD biosynthetic process from tryptophan Source: GO_Central
Anthranilate metabolic process Source: UniProtKB-UniRule
NAD biosynthetic process Source: UniProtKB
Neuron cellular homeostasis Source: UniProtKB
Quinolinate biosynthetic process Source: UniProtKB
Quinolinate metabolic process Source: GO_Central
Response to cadmium ion Source: UniProtKB
Response to zinc ion Source: UniProtKB
Tryptophan catabolic process Source: UniProtKB-UniRule

Cellular Location

Cytosol

Involvement in disease

Vertebral, cardiac, renal, and limb defects syndrome 1 (VCRL1):
An autosomal recessive congenital malformation syndrome characterized by vertebral segmentation abnormalities, congenital cardiac defects, renal defects, and distal mild limb defects.

Sanz, I., Altomare, A., Mondanelli, G., Protti, M., Valsecchi, V., Mercolini, L., ... & Regazzoni, L. (2022). Chromatographic measurement of 3-hydroxyanthranilate 3, 4-dioxygenase activity reveals that edaravone can mitigate the formation of quinolinic acid through a direct enzyme inhibition. Journal of Pharmaceutical and Biomedical Analysis, 219, 114948.

Wang, Y., Liu, K. F., Yang, Y., Davis, I., & Liu, A. (2020). Observing 3-hydroxyanthranilate-3, 4-dioxygenase in action through a crystalline lens. Proceedings of the National Academy of Sciences, 117(33), 19720-19730.

Yang, Y., Liu, F., & Liu, A. (2018). Adapting to oxygen: 3-Hydroxyanthrinilate 3, 4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities. Journal of Biological Chemistry, 293(27), 10415-10424.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant HAAO protein, GST Tag (CAT#: V2LY-0526-LY4448)

Human HAAO ELISA Kit (CAT#: V2LY-0626-LY4297)

Related Products

Mouse Anti-HAAO Recombinant Antibody (CBFYH-0645) (CAT#: CBMAB-H0551-FY)

Human HAAO ELISA Kit (CAT#: V2LY-0626-LY4297)

Human Recombinant HAAO protein, GST Tag (CAT#: V2LY-0526-LY4448)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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