Mouse Anti-HAO1 Recombinant Antibody (EG1475) (CBMAB-EN1762-LY)

Name: Mouse Anti-HAO1 Recombinant Antibody (EG1475)
SKU: CBMAB-EN1762-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG1475

Application

WB, IF, IHC-p

Immunogen

Recombinant Protein of HAO1

Specificity

Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

hydroxyacid oxidase 1, liver

Entrez Gene ID

Mouse	15112
Rat	311446

UniProt ID

Mouse	Q9WU19
Rat	B0BNF9

Alternative Names

GO; GOX; Hao; Gox1; Hao-1

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed:10978532, PubMed:17669354).

Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed:10777549, PubMed:17669354, PubMed:18215067).

Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

Is not active on L-lactate and 2-hydroxybutanoate (PubMed:10777549).

Biological Process

Fatty acid alpha-oxidation Source: UniProtKB
Glycolate catabolic process Source: UniProtKB
Response to oxidative stress Source: Ensembl

Cellular Location

Peroxisome matrix

Zeng, Z., Xu, S., Wang, F., Peng, X., Zhang, W., Zhan, Y., ... & Liang, L. (2022). HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps. Oncogene, 41(29), 3719-3731.

Kimura, A., Hirayama, A., Matsumoto, T., Sato, Y., Kobayashi, T., Ikeda, S., ... & Miyamoto, T. (2022). Hao1 is not a pathogenic factor for ectopic ossifications but functions to regulate the TCA cycle in vivo. Metabolites, 12(1), 82.

Chen, H., Li, X., Sun, Y., Du, Y., Wu, S., Wu, Y., ... & Yin, S. (2022). HAO1 negatively regulates liver macrophage activation via the NF-κB pathway in alcohol-associated liver disease. Cellular Signalling, 99, 110436.

Lee, E. C., McRiner, A. J., Georgiadis, K. E., Liu, J., Wang, Z., Ferguson, A. D., ... & Kohlmann, A. (2021). Discovery of novel, potent inhibitors of hydroxy acid oxidase 1 (HAO1) using DNA-encoded chemical library screening. Journal of Medicinal Chemistry, 64(10), 6730-6744.

McGregor, T. L., Hunt, K. A., Nioi, P., Mason, D., Ticau, S., Pelosi, M., ... & van Heel, D. A. (2019). Deep phenotyping of a healthy human HAO1 knockout informs therapeutic development for primary hyperoxaluria type 1. BioRxiv, 524256.

MacKinnon, S., Arruda, B., Krojer, T., Bradley, A. R., Talon, R., Brandao-Neto, J., ... & Yue, W. W. (2018). Human hydroxyacid oxidase (HAO1); a target enabling package.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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