Human Recombinant HAO1 protein, His Tag (V2LY-0526-LY4454)

Name: Human Recombinant HAO1 protein, His Tag
SKU: V2LY-0526-LY4454
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant HAO1 protein, His Tag consist of Amino Acid: 2-370 and predicts a molecular mass of 42 kDa.

Molecule Mass

42 kDa

Sequence

Amino Acid: 2-370

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

hydroxyacid oxidase 1, liver

Alternative Names

GO; GOX; Hao; Gox1; Hao-1

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed:10978532, PubMed:17669354).

Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed:10777549, PubMed:17669354, PubMed:18215067).

Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

Is not active on L-lactate and 2-hydroxybutanoate (PubMed:10777549).

Biological Process

Fatty acid alpha-oxidation Source: UniProtKB
Glycolate catabolic process Source: UniProtKB
Response to oxidative stress Source: Ensembl

Cellular Location

Peroxisome matrix

Zeng, Z., Xu, S., Wang, F., Peng, X., Zhang, W., Zhan, Y., ... & Liang, L. (2022). HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps. Oncogene, 41(29), 3719-3731.

Kimura, A., Hirayama, A., Matsumoto, T., Sato, Y., Kobayashi, T., Ikeda, S., ... & Miyamoto, T. (2022). Hao1 is not a pathogenic factor for ectopic ossifications but functions to regulate the TCA cycle in vivo. Metabolites, 12(1), 82.

Chen, H., Li, X., Sun, Y., Du, Y., Wu, S., Wu, Y., ... & Yin, S. (2022). HAO1 negatively regulates liver macrophage activation via the NF-κB pathway in alcohol-associated liver disease. Cellular Signalling, 99, 110436.

Lee, E. C., McRiner, A. J., Georgiadis, K. E., Liu, J., Wang, Z., Ferguson, A. D., ... & Kohlmann, A. (2021). Discovery of novel, potent inhibitors of hydroxy acid oxidase 1 (HAO1) using DNA-encoded chemical library screening. Journal of Medicinal Chemistry, 64(10), 6730-6744.

McGregor, T. L., Hunt, K. A., Nioi, P., Mason, D., Ticau, S., Pelosi, M., ... & van Heel, D. A. (2019). Deep phenotyping of a healthy human HAO1 knockout informs therapeutic development for primary hyperoxaluria type 1. BioRxiv, 524256.

MacKinnon, S., Arruda, B., Krojer, T., Bradley, A. R., Talon, R., Brandao-Neto, J., ... & Yue, W. W. (2018). Human hydroxyacid oxidase (HAO1); a target enabling package.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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