Human HAO1 ELISA Kit (V2LY-0626-LY4703)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
40.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
hydroxyacid oxidase 1, liver
Alternative Names
GO; GOX; Hao; Gox1; Hao-1
Function
Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed:10978532, PubMed:17669354).

Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed:10777549, PubMed:17669354, PubMed:18215067).

Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

Is not active on L-lactate and 2-hydroxybutanoate (PubMed:10777549).
Biological Process
Fatty acid alpha-oxidation Source: UniProtKB
Glycolate catabolic process Source: UniProtKB
Response to oxidative stress Source: Ensembl
Cellular Location
Peroxisome matrix

Zeng, Z., Xu, S., Wang, F., Peng, X., Zhang, W., Zhan, Y., ... & Liang, L. (2022). HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps. Oncogene, 41(29), 3719-3731.

Kimura, A., Hirayama, A., Matsumoto, T., Sato, Y., Kobayashi, T., Ikeda, S., ... & Miyamoto, T. (2022). Hao1 is not a pathogenic factor for ectopic ossifications but functions to regulate the TCA cycle in vivo. Metabolites, 12(1), 82.

Chen, H., Li, X., Sun, Y., Du, Y., Wu, S., Wu, Y., ... & Yin, S. (2022). HAO1 negatively regulates liver macrophage activation via the NF-κB pathway in alcohol-associated liver disease. Cellular Signalling, 99, 110436.

Lee, E. C., McRiner, A. J., Georgiadis, K. E., Liu, J., Wang, Z., Ferguson, A. D., ... & Kohlmann, A. (2021). Discovery of novel, potent inhibitors of hydroxy acid oxidase 1 (HAO1) using DNA-encoded chemical library screening. Journal of Medicinal Chemistry, 64(10), 6730-6744.

McGregor, T. L., Hunt, K. A., Nioi, P., Mason, D., Ticau, S., Pelosi, M., ... & van Heel, D. A. (2019). Deep phenotyping of a healthy human HAO1 knockout informs therapeutic development for primary hyperoxaluria type 1. BioRxiv, 524256.

MacKinnon, S., Arruda, B., Krojer, T., Bradley, A. R., Talon, R., Brandao-Neto, J., ... & Yue, W. W. (2018). Human hydroxyacid oxidase (HAO1); a target enabling package.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant HAO1 protein, His Tag (CAT#: V2LY-0526-LY4454)

Mouse Hao1 ELISA Kit (CAT#: V2LY-0626-LY5819)

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Isotype control

For research use only. Not intended for any clinical use.

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