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Mouse Anti-HAO1 Recombinant Antibody (CBFYH-0681) (CBMAB-H1571-FY)

This product is mouse antibody that recognizes HAO1. The antibody CBFYH-0681 can be used for immunoassay techniques such as: WB, IF, FC.
See all HAO1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0681
Antibody Isotype
IgG1
Application
WB, IF, FC

Basic Information

Immunogen
Full length human recombinant protein of human HAO1 produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, PH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
hydroxyacid oxidase 1, liver
Introduction
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
Entrez Gene ID
54363
UniProt ID
Q9UJM8
Alternative Names
Hydroxyacid Oxidase 1; Hydroxyacid Oxidase (Glycolate Oxidase) 1; Glycolate Oxidase 1; HAOX1; GOX1; GOX; (S)-2-Hydroxy-Acid Oxidase; Glycolate Oxidase; EC 1.1.3.15
Function
Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed:10978532, PubMed:17669354).

Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed:10777549, PubMed:17669354, PubMed:18215067).

Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:10777549, PubMed:17669354, PubMed:18215067, PubMed:10978532).

Is not active on L-lactate and 2-hydroxybutanoate (PubMed:10777549).
Biological Process
Fatty acid alpha-oxidation Source: UniProtKB
Glycolate catabolic process Source: UniProtKB
Response to oxidative stress Source: Ensembl
Cellular Location
Peroxisome matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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