Mouse Anti-HIPK4 Recombinant Antibody (2E8) (CBMAB-A3914-LY)

The product is antibody recognizes HIPK4. The antibody 2E8 immunoassay techniques such as: WB, ELISA.
See all HIPK4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2E8

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

HIPK4 (AAH34501.1, 519 a.a. ~ 616 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

homeodomain interacting protein kinase 4

Entrez Gene ID

147746

UniProt ID

Q8NE63

Alternative Names

FLJ32818

Function

Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity).

May act as a corepressor of transcription factors (Potential).

Biological Process

Histone phosphorylation Source: Ensembl
Peptidyl-serine phosphorylation Source: GO_Central
Peptidyl-threonine phosphorylation Source: GO_Central
Protein autophosphorylation Source: Ensembl
Regulation of signal transduction by p53 class mediator Source: Ensembl

Cellular Location

Cytoplasm

PTM

Autophosphorylated.

References

Liu, X., Zang, C., Wu, Y., Meng, R., Chen, Y., Jiang, T., ... & Guo, X. (2022). Homeodomain-interacting protein kinase HIPK4 regulates phosphorylation of manchette protein RIMBP3 during spermiogenesis. Journal of Biological Chemistry, 298(9).

Crapster, J. A., Rack, P. G., Hellmann, Z. J., Le, A. D., Adams, C. M., Leib, R. D., ... & Chen, J. K. (2020). HIPK4 is essential for murine spermiogenesis. elife, 9, e50209.

Aaron, C. J., Rack, P. G., Hellmann, Z. J., Le, A. D., Adams, C. M., Leib, R. D., ... & Chen, J. K. (2020). HIPK4 is essential for murine spermiogenesis. eLife, 9.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

HIPK4 Matched Antibody Pair (543)

Related Products

Mouse Anti-HIPK4 Recombinant Antibody (CBFYH-1135)

HIPK4 Matched Antibody Pair (543)

Rabbit Anti-HIPK4 Recombinant Antibody (EG1513)

Mouse Anti-HIPK4 Monoclonal Antibody (CBFYH-3072)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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