Mouse Anti-HLX Recombinant Antibody (CBT727) (V2LY-0625-LY3325)

Name: Mouse Anti-HLX Recombinant Antibody (CBT727)
SKU: V2LY-0625-LY3325
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-HLX Recombinant Antibody (CBT727) (V2LY-0625-LY3325) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT727

Application

Immunogen

Purified recombinant human HLX1 (C-terminus) protein fragments expressed in E.coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog, Pig

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

H2.0 like homeobox

Entrez Gene ID

3142

UniProt ID

Q14774

Function

Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis (By similarity).

Biological Process

Cell differentiation Source: UniProtKB-KW
Embryonic digestive tract morphogenesis Source: Ensembl
Enteric nervous system development Source: Ensembl
Liver development Source: Ensembl
Multicellular organism development Source: ProtInc
Negative regulation of T-helper 2 cell differentiation Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of organ growth Source: Ensembl
Positive regulation of T-helper 1 cell differentiation Source: Ensembl
Skeletal muscle tissue development Source: Ensembl

Cellular Location

Nucleus

Zhu, X. Y., Guo, Q. Y., Zhu, M., Chen, B. G., Wang, L. Y., Zhang, D. Q., ... & Luo, W. D. (2020). HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway. Oncology Letters, 20(2), 1888-1896.

Piragyte, I., Clapes, T., Polyzou, A., Klein Geltink, R. I., Lefkopoulos, S., Yin, N., ... & Trompouki, E. (2018). A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways. Nature communications, 9(1), 3090.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-HLX (AA 176-279) Recombinant Antibody (CBFYH-1486) (CAT#: CBMAB-H2441-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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