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Mouse Anti-IDH2 Recombinant Antibody (2G10) (CBMAB-A4219-LY)

The product is antibody recognizes IDH2. The antibody 2G10 immunoassay techniques such as: WB, ELISA.
See all IDH2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G10
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
IDH2 (NP_002159, 354 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial
Introduction
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]
Entrez Gene ID
3418
UniProt ID
P48735
Alternative Names
ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Biological Process
2-oxoglutarate metabolic process Source: UniProtKB
Carbohydrate metabolic process Source: ProtInc
Glyoxylate cycle Source: UniProtKB-KW
Isocitrate metabolic process Source: UniProtKB
NADP metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: UniProtKB-KW
Cellular Location
Mitochondrion
Involvement in disease
D-2-hydroxyglutaric aciduria 2 (D2HGA2):
A neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.
Glioma (GLM):
Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
PTM
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

Watany, M. M., Abd-Ellatif, R. N., Abdeldayem, M. E., & El-Horany, H. E. S. (2022). Association between genetic variations of mitochondrial isocitrate dehydrogenase (IDH2) and acute myocardial infarction. Gene, 829, 146497.

Murugan, A. K., & Alzahrani, A. S. (2022). Isocitrate dehydrogenase IDH1 and IDH2 mutations in human cancer: prognostic implications for gliomas. British Journal of Biomedical Science, 79, 10208.

Lang, F., Jha, A., Meuter, L., Pacak, K., & Yang, C. (2021). Identification of isocitrate dehydrogenase 2 (IDH2) mutation in carotid body paraganglioma. Frontiers in Endocrinology, 12, 731096.

Noh, M. R., Kong, M. J., Han, S. J., Kim, J. I., & Park, K. M. (2020). Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension. Redox Biology, 34, 101548.

Smolková, K., Špačková, J., Gotvaldová, K., Dvořák, A., Křenková, A., Hubálek, M., ... & Ježek, P. (2020). SIRT3 and GCN5L regulation of NADP+-and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2. Scientific Reports, 10(1), 8677.

Aljohani, A. I., Toss, M. S., Kurozumi, S., Joseph, C., Aleskandarany, M. A., Miligy, I. M., ... & Rakha, E. A. (2020). The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast cancer research and treatment, 179, 79-90.

Kong, M. J., Han, S. J., Kim, J. I., Park, J. W., & Park, K. M. (2018). Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells. Cell Death & Disease, 9(5), 488.

Kolb, A. L., Corridon, P. R., Zhang, S., Xu, W., Witzmann, F. A., Collett, J. A., ... & Bacallao, R. L. (2018). Exogenous gene transmission of isocitrate dehydrogenase 2 mimics ischemic preconditioning protection. Journal of the American Society of Nephrology: JASN, 29(4), 1154.

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For research use only. Not intended for any clinical use.

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