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Mouse Anti-IDH2 Recombinant Antibody (CBT4722) (V2LY-0625-LY1230)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4722
Antibody Isotype
IgG1
Application
IHC, FC

Basic Information

Immunogen
Purified recombinant fragment of human IDH2 (AA: 1-143) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial
Entrez Gene ID
3418
UniProt ID
P48735
Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Biological Process
2-oxoglutarate metabolic process Source: UniProtKB
Carbohydrate metabolic process Source: ProtInc
Glyoxylate cycle Source: UniProtKB-KW
Isocitrate metabolic process Source: UniProtKB
NADP metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: UniProtKB-KW
Cellular Location
Mitochondrion
Involvement in disease
D-2-hydroxyglutaric aciduria 2 (D2HGA2):
A neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.
Glioma (GLM):
Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
PTM
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
More Infomation

Watany, M. M., Abd-Ellatif, R. N., Abdeldayem, M. E., & El-Horany, H. E. S. (2022). Association between genetic variations of mitochondrial isocitrate dehydrogenase (IDH2) and acute myocardial infarction. Gene, 829, 146497.

Murugan, A. K., & Alzahrani, A. S. (2022). Isocitrate dehydrogenase IDH1 and IDH2 mutations in human cancer: prognostic implications for gliomas. British Journal of Biomedical Science, 79, 10208.

Lang, F., Jha, A., Meuter, L., Pacak, K., & Yang, C. (2021). Identification of isocitrate dehydrogenase 2 (IDH2) mutation in carotid body paraganglioma. Frontiers in Endocrinology, 12, 731096.

Noh, M. R., Kong, M. J., Han, S. J., Kim, J. I., & Park, K. M. (2020). Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension. Redox Biology, 34, 101548.

Smolková, K., Špačková, J., Gotvaldová, K., Dvořák, A., Křenková, A., Hubálek, M., ... & Ježek, P. (2020). SIRT3 and GCN5L regulation of NADP+-and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2. Scientific Reports, 10(1), 8677.

Aljohani, A. I., Toss, M. S., Kurozumi, S., Joseph, C., Aleskandarany, M. A., Miligy, I. M., ... & Rakha, E. A. (2020). The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast cancer research and treatment, 179, 79-90.

Kong, M. J., Han, S. J., Kim, J. I., Park, J. W., & Park, K. M. (2018). Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells. Cell Death & Disease, 9(5), 488.

Kolb, A. L., Corridon, P. R., Zhang, S., Xu, W., Witzmann, F. A., Collett, J. A., ... & Bacallao, R. L. (2018). Exogenous gene transmission of isocitrate dehydrogenase 2 mimics ischemic preconditioning protection. Journal of the American Society of Nephrology: JASN, 29(4), 1154.

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For research use only. Not intended for any clinical use.

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