Human IDH2 ELISA Kit (V2LY-0626-LY3627)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.3 ng/mL
Detection Range
0.5-300 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
50.9 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial
Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Biological Process
2-oxoglutarate metabolic process Source: UniProtKB
Carbohydrate metabolic process Source: ProtInc
Glyoxylate cycle Source: UniProtKB-KW
Isocitrate metabolic process Source: UniProtKB
NADP metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: UniProtKB-KW
Cellular Location
Mitochondrion
Involvement in disease
D-2-hydroxyglutaric aciduria 2 (D2HGA2):
A neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.
Glioma (GLM):
Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
PTM
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

Watany, M. M., Abd-Ellatif, R. N., Abdeldayem, M. E., & El-Horany, H. E. S. (2022). Association between genetic variations of mitochondrial isocitrate dehydrogenase (IDH2) and acute myocardial infarction. Gene, 829, 146497.

Murugan, A. K., & Alzahrani, A. S. (2022). Isocitrate dehydrogenase IDH1 and IDH2 mutations in human cancer: prognostic implications for gliomas. British Journal of Biomedical Science, 79, 10208.

Lang, F., Jha, A., Meuter, L., Pacak, K., & Yang, C. (2021). Identification of isocitrate dehydrogenase 2 (IDH2) mutation in carotid body paraganglioma. Frontiers in Endocrinology, 12, 731096.

Noh, M. R., Kong, M. J., Han, S. J., Kim, J. I., & Park, K. M. (2020). Isocitrate dehydrogenase 2 deficiency aggravates prolonged high-fat diet intake-induced hypertension. Redox Biology, 34, 101548.

Smolková, K., Špačková, J., Gotvaldová, K., Dvořák, A., Křenková, A., Hubálek, M., ... & Ježek, P. (2020). SIRT3 and GCN5L regulation of NADP+-and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2. Scientific Reports, 10(1), 8677.

Aljohani, A. I., Toss, M. S., Kurozumi, S., Joseph, C., Aleskandarany, M. A., Miligy, I. M., ... & Rakha, E. A. (2020). The prognostic significance of wild-type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast cancer research and treatment, 179, 79-90.

Kong, M. J., Han, S. J., Kim, J. I., Park, J. W., & Park, K. M. (2018). Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells. Cell Death & Disease, 9(5), 488.

Kolb, A. L., Corridon, P. R., Zhang, S., Xu, W., Witzmann, F. A., Collett, J. A., ... & Bacallao, R. L. (2018). Exogenous gene transmission of isocitrate dehydrogenase 2 mimics ischemic preconditioning protection. Journal of the American Society of Nephrology: JASN, 29(4), 1154.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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