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Rabbit Anti-IFIT1 Recombinant Antibody (D2X9Z) (CBMAB-CP1095-LY)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
D2X9Z
Application
WB, IP, IHC, IHC-P
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Interferon Induced Protein With Tetratricopeptide Repeats 1
Introduction
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]
Entrez Gene ID
64135
UniProt ID
Q9BYX4
Alternative Names
Interferon Induced With Helicase C Domain 1
Function
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
Biological Process
Antiviral innate immune response Source: ARUK-UCL
Cellular response to exogenous dsRNA Source: BHF-UCL
Cellular response to type I interferon Source: BHF-UCL
Defense response to virus Source: GO_Central
Intracellular transport of viral protein in host cell Source: BHF-UCL
Negative regulation of helicase activity Source: BHF-UCL
Negative regulation of protein binding Source: BHF-UCL
Negative regulation of viral genome replication Source: UniProtKB
Positive regulation of viral genome replication Source: BHF-UCL
Regulation of defense response to virus Source: InterPro
Response to virus Source: BHF-UCL
Cellular Location
Cytoplasm
PTM
Phosphorylated.
ISGylated.

Gao, Y., Zou, T., Xu, P., Wang, Y., Jiang, Y., Chen, Y. X., ... & Fang, J. Y. (2023). Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer. Neoplasia, 35, 100850.

Schindewolf, C., Lokugamage, K., Vu, M. N., Johnson, B. A., Scharton, D., Plante, J. A., ... & Menachery, V. D. (2023). SARS-CoV-2 uses nonstructural protein 16 to evade restriction by IFIT1 and IFIT3. Journal of virology, 97(2), e01532-22.

Russ, A., Wittmann, S., Tsukamoto, Y., Herrmann, A., Deutschmann, J., Lagisquet, J., ... & Gramberg, T. (2022). Nsp16 shields SARS–CoV‐2 from efficient MDA5 sensing and IFIT1‐mediated restriction. EMBO reports, 23(12), e55648.

Zhang, M., Zhu, Y., Zhu, J., Xie, Y., Wu, R., Zhong, J., ... & Jiang, L. (2022). circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3p. Cellular & Molecular Biology Letters, 27(1), 1-26.

Liu, G., Sun, J., Yang, Z. F., Zhou, C., Zhou, P. Y., Guan, R. Y., ... & Yi, Y. (2021). Cancer-associated fibroblast-derived CXCL11 modulates hepatocellular carcinoma cell migration and tumor metastasis through the circUBAP2/miR-4756/IFIT1/3 axis. Cell death & disease, 12(3), 260.

Li, T. H., Zhao, B. B., Qin, C., Wang, Y. Y., Li, Z. R., Cao, H. T., ... & Wang, W. B. (2021). IFIT1 modulates the proliferation, migration and invasion of pancreatic cancer cells via Wnt/β-catenin signaling. Cellular Oncology, 44(6), 1425-1437.

Pidugu, V. K., Wu, M. M., Yen, A. H., Pidugu, H. B., Chang, K. W., Liu, C. J., & Lee, T. C. (2019). IFIT1 and IFIT3 promote oral squamous cell carcinoma metastasis and contribute to the anti-tumor effect of gefitinib via enhancing p-EGFR recycling. Oncogene, 38(17), 3232-3247.

Johnson, B., VanBlargan, L. A., Xu, W., White, J. P., Shan, C., Shi, P. Y., ... & Amarasinghe, G. K. (2018). Human IFIT3 modulates IFIT1 RNA binding specificity and protein stability. Immunity, 48(3), 487-499.

Fleith, R. C., Mears, H. V., Leong, X. Y., Sanford, T. J., Emmott, E., Graham, S. C., ... & Sweeney, T. R. (2018). IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. Nucleic acids research, 46(10), 5269-5285.

John, S. P., Sun, J., Carlson, R. J., Cao, B., Bradfield, C. J., Song, J., ... & Fraser, I. D. (2018). IFIT1 exerts opposing regulatory effects on the inflammatory and interferon gene programs in LPS-activated human macrophages. Cell reports, 25(1), 95-106.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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