Human IFIT1 ELISA Kit (V2LY-0626-LY4749)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
55.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interferon Induced Protein With Tetratricopeptide Repeats 1
Function
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
Biological Process
Antiviral innate immune response Source: ARUK-UCL
Cellular response to exogenous dsRNA Source: BHF-UCL
Cellular response to type I interferon Source: BHF-UCL
Defense response to virus Source: GO_Central
Intracellular transport of viral protein in host cell Source: BHF-UCL
Negative regulation of helicase activity Source: BHF-UCL
Negative regulation of protein binding Source: BHF-UCL
Negative regulation of viral genome replication Source: UniProtKB
Positive regulation of viral genome replication Source: BHF-UCL
Regulation of defense response to virus Source: InterPro
Response to virus Source: BHF-UCL
Cellular Location
Cytoplasm
PTM
Phosphorylated.
ISGylated.

Gao, Y., Zou, T., Xu, P., Wang, Y., Jiang, Y., Chen, Y. X., ... & Fang, J. Y. (2023). Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer. Neoplasia, 35, 100850.

Schindewolf, C., Lokugamage, K., Vu, M. N., Johnson, B. A., Scharton, D., Plante, J. A., ... & Menachery, V. D. (2023). SARS-CoV-2 uses nonstructural protein 16 to evade restriction by IFIT1 and IFIT3. Journal of virology, 97(2), e01532-22.

Russ, A., Wittmann, S., Tsukamoto, Y., Herrmann, A., Deutschmann, J., Lagisquet, J., ... & Gramberg, T. (2022). Nsp16 shields SARS–CoV‐2 from efficient MDA5 sensing and IFIT1‐mediated restriction. EMBO reports, 23(12), e55648.

Zhang, M., Zhu, Y., Zhu, J., Xie, Y., Wu, R., Zhong, J., ... & Jiang, L. (2022). circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3p. Cellular & Molecular Biology Letters, 27(1), 1-26.

Liu, G., Sun, J., Yang, Z. F., Zhou, C., Zhou, P. Y., Guan, R. Y., ... & Yi, Y. (2021). Cancer-associated fibroblast-derived CXCL11 modulates hepatocellular carcinoma cell migration and tumor metastasis through the circUBAP2/miR-4756/IFIT1/3 axis. Cell death & disease, 12(3), 260.

Li, T. H., Zhao, B. B., Qin, C., Wang, Y. Y., Li, Z. R., Cao, H. T., ... & Wang, W. B. (2021). IFIT1 modulates the proliferation, migration and invasion of pancreatic cancer cells via Wnt/β-catenin signaling. Cellular Oncology, 44(6), 1425-1437.

Pidugu, V. K., Wu, M. M., Yen, A. H., Pidugu, H. B., Chang, K. W., Liu, C. J., & Lee, T. C. (2019). IFIT1 and IFIT3 promote oral squamous cell carcinoma metastasis and contribute to the anti-tumor effect of gefitinib via enhancing p-EGFR recycling. Oncogene, 38(17), 3232-3247.

Johnson, B., VanBlargan, L. A., Xu, W., White, J. P., Shan, C., Shi, P. Y., ... & Amarasinghe, G. K. (2018). Human IFIT3 modulates IFIT1 RNA binding specificity and protein stability. Immunity, 48(3), 487-499.

Fleith, R. C., Mears, H. V., Leong, X. Y., Sanford, T. J., Emmott, E., Graham, S. C., ... & Sweeney, T. R. (2018). IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. Nucleic acids research, 46(10), 5269-5285.

John, S. P., Sun, J., Carlson, R. J., Cao, B., Bradfield, C. J., Song, J., ... & Fraser, I. D. (2018). IFIT1 exerts opposing regulatory effects on the inflammatory and interferon gene programs in LPS-activated human macrophages. Cell reports, 25(1), 95-106.

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For research use only. Not intended for any clinical use.

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