Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711) (V2LY-0625-LY1954)

Name: Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711)
SKU: V2LY-0625-LY1954
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711) (V2LY-0625-LY1954) in ELISA

Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711) (V2LY-0625-LY1954) in WB

Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711) (V2LY-0625-LY1954) in FC

Mouse Anti-IFNAR1 Recombinant Antibody (CBT4711) (V2LY-0625-LY1954) in IHC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4711

Application

WB, IHC, FC

Immunogen

Purified recombinant fragment of human IFNAR1 (AA: 28-156) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Interferon Alpha And Beta Receptor Subunit 1

Entrez Gene ID

3454

UniProt ID

P17181

Alternative Names

Interferon Alpha And Beta Receptor Subunit 1

Function

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986).

Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986).

Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (PubMed:7665574, PubMed:21854986, PubMed:32972995).

Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).

Biological Process

Cellular response to interferon-alpha Source: UniProtKB
Cytokine-mediated signaling pathway Source: GO_Central
Positive regulation of cellular respiration Source: ARUK-UCL
Receptor signaling pathway via JAK-STAT Source: ProtInc
Response to lipopolysaccharide Source: UniProtKB
Response to virus Source: ProtInc
Type I interferon signaling pathway Source: UniProtKB

Cellular Location

Isoform 1: Lysosome; Cell membrane; Late endosome. Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.

Topology

Extracellular: 28-436
Helical: 437-457
Cytoplasmic: 458-557

PTM

Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985).
Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744).
Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

Abolhassani, H., Landegren, N., Bastard, P., Materna, M., Modaresi, M., Du, L., ... & Pan-Hammarström, Q. (2022). Inherited IFNAR1 deficiency in a child with both critical COVID-19 pneumonia and multisystem inflammatory syndrome. Journal of Clinical Immunology, 42(3), 471-483.

Khanmohammadi, S., Rezaei, N., Khazaei, M., & Shirkani, A. (2022). A case of autosomal recessive interferon alpha/beta receptor alpha chain (IFNAR1) deficiency with severe COVID-19. Journal of clinical immunology, 1-6.

Bastard, P., Hsiao, K. C., Zhang, Q., Choin, J., Best, E., Chen, J., ... & Casanova, J. L. (2022). A loss-of-function IFNAR1 allele in Polynesia underlies severe viral diseases in homozygotes. Journal of Experimental Medicine, 219(6), e20220028.

Bastard, P., Manry, J., Chen, J., Rosain, J., Seeleuthner, Y., AbuZaitun, O., ... & Zhang, S. Y. (2021). Herpes simplex encephalitis in a patient with a distinctive form of inherited IFNAR1 deficiency. The Journal of clinical investigation, 131(1).

Xu, F., Wang, H., Tian, J., & Xu, H. (2021). Down-regulation of ID2-AS1 alleviates the neuronal injury induced by 1-methy1-4-phenylpyridinium in human neuroblastoma cell line SH-SY5Y cells through regulating miR-199a-5p/IFNAR1/JAK2/STAT1 Axis. Neurochemical Research, 46, 2192-2203.

Shemesh, M., Lochte, S., Piehler, J., & Schreiber, G. (2021). IFNAR1 and IFNAR2 play distinct roles in initiating type I interferon–induced JAK-STAT signaling and activating STATs. Science signaling, 14(710), eabe4627.

Saminathan, M., Singh, K. P., Vineetha, S., Maity, M., Biswas, S. K., Manjunathareddy, G. B., ... & Mertens, P. P. C. (2020). Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid gestation. Scientific Reports, 10(1), 2164.

Tian, J., Kang, H., Huang, J., Li, Z., Pan, Y., Li, Y., ... & Qu, L. (2020). Feline calicivirus strain 2280 p30 antagonizes type I interferon-mediated antiviral innate immunity through directly degrading IFNAR1 mRNA. PLoS Pathogens, 16(10), e1008944.

Hernandez, N., Bucciol, G., Moens, L., Le Pen, J., Shahrooei, M., Goudouris, E., ... & Bossuyt, X. (2019). Inherited IFNAR1 deficiency in otherwise healthy patients with adverse reaction to measles and yellow fever live vaccines. Journal of Experimental Medicine, 216(9), 2057-2070.

Zhang, G., Deweerd, N. A., Stifter, S. A., Liu, L., Zhou, B., Wang, W., ... & Feng, C. G. (2018). A proline deletion in IFNAR1 impairs IFN-signaling and underlies increased resistance to tuberculosis in humans. Nature communications, 9(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

IFNAR1 Matched Antibody Pair (604) (CAT#: APMAB-604LY)

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Isotype control

For research use only. Not intended for any clinical use.

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