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Mouse Anti-KDM2B Recombinant Antibody (3B7D11) (CBMAB-K0070-LY)

This product is antibody recognizes KDM2B. The antibody 3B7D11 immunoassay techniques such as: WB, FC, ELISA, IHC, ICC.
See all KDM2B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B7D11
Antibody Isotype
IgG
Application
WB, FC, ELISA, IHC, ICC

Basic Information

Immunogen
Purified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Lysine Demethylase 2B
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Entrez Gene ID
84678
UniProt ID
Q8NHM5
Alternative Names
Lysine Demethylase 2B; F-Box And Leucine-Rich Repeat Protein 10; JmjC Domain-Containing Histone Demethylation Protein 1B; Jumonji C Domain-Containing Histone Demethylase 1B; [Histone-H3]-Lysine-36 Demethylase 1B; Lysine (K)-Specific Demethylase 2B; Protein-Containing CXXC Domain 2; CXXC-Type Zinc Finger Protein 2; F-Box/LRR-Repeat Protein 10; F-Box Protein FBL10; EC 1.14.11.27;
Function
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code (PubMed:16362057, PubMed:17994099, PubMed:26237645).
Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36' (PubMed:16362057, PubMed:17994099, PubMed:26237645).
Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation (PubMed:16362057, PubMed:17994099).
May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (Probable)
Biological Process
Chromatin organizationIEA:UniProtKB-KW
Embryonic camera-type eye morphogenesisISS:BHF-UCL
Forebrain developmentISS:BHF-UCL
Fourth ventricle developmentISS:BHF-UCL
Hindbrain developmentISS:BHF-UCL
Histone H2A monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Initiation of neural tube closureISS:BHF-UCL
Lateral ventricle developmentISS:BHF-UCL
Midbrain developmentISS:BHF-UCL
Midbrain-hindbrain boundary morphogenesisISS:BHF-UCL
Negative regulation of neural precursor cell proliferationISS:BHF-UCL
Negative regulation of neuron apoptotic processISS:BHF-UCL
Negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Positive regulation of cell growthIEA:Ensembl
Positive regulation of stem cell population maintenanceIEA:Ensembl
Protein demethylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
SpermatogenesisISS:BHF-UCL
Third ventricle developmentISS:BHF-UCL
Cellular Location
Nucleus, nucleolus; Nucleus; Chromosome

Wanna-Udom, S., Terashima, M., Suphakhong, K., Ishimura, A., Takino, T., & Suzuki, T. (2021). KDM2B is involved in the epigenetic regulation of TGF-β-induced epithelial–mesenchymal transition in lung and pancreatic cancer cell lines. Journal of Biological Chemistry, 296.

Sanches, J. G. P., Song, B., Zhang, Q., Cui, X., Yabasin, I. B., Ntim, M., ... & Li, L. (2021). The role of KDM2B and EZH2 in regulating the stemness in colorectal cancer through the PI3K/AKT pathway. Frontiers in Oncology, 11, 637298.

Kuang, Y., Xu, H., Lu, F., Meng, J., Yi, Y., Yang, H., ... & Su, S. (2021). Inhibition of microRNA let‐7b expression by KDM2B promotes cancer progression by targeting EZH2 in ovarian cancer. Cancer Science, 112(1), 231-242.

Quan, M., Chen, Z., Jiao, F., Xiao, X., Xia, Q., Chen, J., ... & Cui, J. (2020). Lysine demethylase 2 (KDM2B) regulates hippo pathway via MOB1 to promote pancreatic ductal adenocarcinoma (PDAC) progression. Journal of Experimental & Clinical Cancer Research, 39(1), 1-15.

Zhou, Q., Zhang, Y., Wang, B., Zhou, W., Bi, Y., Huai, W., ... & Zhan, Z. (2020). KDM2B promotes IL-6 production and inflammatory responses through Brg1-mediated chromatin remodeling. Cellular & molecular immunology, 17(8), 834-842.

Isshiki, Y., Nakajima-Takagi, Y., Oshima, M., Aoyama, K., Rizk, M., Kurosawa, S., ... & Iwama, A. (2019). KDM2B in polycomb repressive complex 1.1 functions as a tumor suppressor in the initiation of T-cell leukemogenesis. Blood advances, 3(17), 2537-2549.

Zheng, Q., Fan, H., Meng, Z., Yuan, L., Liu, C., Peng, Y., ... & Feng, J. (2018). Histone demethylase KDM2B promotes triple negative breast cancer proliferation by suppressing p15INK4B, p16INK4A, and p57KIP2 transcription. Acta biochimica et biophysica Sinica, 50(9), 897-904.

Yan, M., Yang, X., Wang, H., & Shao, Q. (2018). The critical role of histone lysine demethylase KDM2B in cancer. American journal of translational research, 10(8), 2222.

Wang, Y., Zang, J., Zhang, D., Sun, Z., Qiu, B., & Wang, X. (2018). KDM2B overexpression correlates with poor prognosis and regulates glioma cell growth. OncoTargets and therapy, 201-209.

Zacharopoulou, N., Tsapara, A., Kallergi, G., Schmid, E., Tsichlis, P. N., Kampranis, S. C., & Stournaras, C. (2018). The epigenetic factor KDM2B regulates cell adhesion, small rho GTPases, actin cytoskeleton and migration in prostate cancer cells. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1865(4), 587-597.

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For research use only. Not intended for any clinical use.

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