Mouse Anti-KHK Recombinant Antibody (3D1) (CBMAB-K0811-LY)

Name: Mouse Anti-KHK Recombinant Antibody (3D1)
SKU: CBMAB-K0811-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3D1

Application

FC, IF, IHC, WB

Immunogen

KHK antibody was raised in mouse using a full length recombinant protein of human KHK (NP_000212) produced in HEK293T cells, as the immunogen

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.5 mg-1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ketohexokinase

Introduction

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Entrez Gene ID

3795

UniProt ID

P50053

Alternative Names

Ketohexokinase; Hepatic Fructokinase; Fructokinase; Testicular Tissue Protein Li 102; Ketohexokinase (Fructokinase); EC 2.7.1.3;

Function

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

Biological Process

Fructose metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of glycogen metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Response to fructoseManual Assertion Based On ExperimentIBA:GO_Central
Response to glucoseManual Assertion Based On ExperimentIBA:GO_Central
Response to insulinManual Assertion Based On ExperimentIBA:GO_Central
Response to sucroseManual Assertion Based On ExperimentIBA:GO_Central
Response to zinc ionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Cytosol; Extracellular exosome

Involvement in disease

Fructosuria (FRUCT):
Benign defect of intermediary metabolism.

Helsley, R. N., Park, S. H., Vekaria, H. J., Sullivan, P. G., Conroy, L. R., Sun, R. C., ... & Softic, S. (2023). Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation. Journal of Hepatology, 79(1), 25-42.

Tee, S. S., Kim, N., Cullen, Q., Eskandari, R., Mamakhanyan, A., Srouji, R. M., ... & Keshari, K. R. (2022). Ketohexokinase-mediated fructose metabolism is lost in hepatocellular carcinoma and can be leveraged for metabolic imaging. Science Advances, 8(14), eabm7985.

Shepherd, E. L., Saborano, R., Northall, E., Matsuda, K., Ogino, H., Yashiro, H., ... & Lalor, P. F. (2021). Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis. JHEP Reports, 3(2), 100217.

Kim, J., Kang, J., Kang, Y. L., Woo, J., Kim, Y., Huh, J., & Park, J. W. (2020). Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 11(1), 5436.

Futatsugi, K., Smith, A. C., Tu, M., Raymer, B., Ahn, K., Coffey, S. B., ... & Magee, T. V. (2020). Discovery of PF-06835919: a potent inhibitor of ketohexokinase (KHK) for the treatment of metabolic disorders driven by the overconsumption of fructose. Journal of Medicinal Chemistry, 63(22), 13546-13560.

Hayasaki, T., Ishimoto, T., Doke, T., Hirayama, A., Soga, T., Furuhashi, K., ... & Kadomatsu, K. (2019). Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase. The Journal of Nutritional Biochemistry, 71, 54-62.

Lanaspa, M. A., Andres-Hernando, A., Orlicky, D. J., Cicerchi, C., Jang, C., Li, N., ... & Tolan, D. R. (2018). Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. The Journal of clinical investigation, 128(6), 2226-2238.

Gao, W., Li, N., Li, Z., Xu, J., & Su, C. (2018). Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma. Biochemical and biophysical research communications, 503(3), 1298-1306.

Miller, C. O., Yang, X., Lu, K., Cao, J., Herath, K., Rosahl, T. W., ... & Akiyama, T. E. (2018). Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects. American Journal of Physiology-Endocrinology and Metabolism, 315(3), E386-E393.

Liu, D., Sterpka, J. A., Vatner, D. F., Bell, M., Murray, S., Bhanot, S., ... & Samuel, V. (2018). Targeting ketohexokinase (KHK) with a novel antisense oligonucleotide (ASO) decreases de novo lipogenesis and improves insulin-mediated whole body glucose metabolism. Diabetes, 67(Supplement_1).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

KHK Matched Antibody Pair (658) (CAT#: APMAB-658LY)

KHK Matched Antibody Pair (659) (CAT#: APMAB-659LY)

KHK Matched Antibody Pair (660) (CAT#: APMAB-660LY)

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Mouse Anti-KHK Recombinant Antibody (2C3) (CAT#: CBMAB-K0809-LY)

KHK Matched Antibody Pair (658) (CAT#: APMAB-658LY)

Mouse Anti-KHK Recombinant Antibody (1F1) (CAT#: CBMAB-K0806-LY)

Isotype control

For research use only. Not intended for any clinical use.

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