Human KHK ELISA Kit (V2LY-0626-LY5925)

Name: Human KHK ELISA Kit
SKU: V2LY-0626-LY5925
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

5.27 mU/mL

Detection Range

10-2000 mU/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

32.5 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Ketohexokinase

Function

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

Biological Process

Fructose metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of glycogen metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Response to fructoseManual Assertion Based On ExperimentIBA:GO_Central
Response to glucoseManual Assertion Based On ExperimentIBA:GO_Central
Response to insulinManual Assertion Based On ExperimentIBA:GO_Central
Response to sucroseManual Assertion Based On ExperimentIBA:GO_Central
Response to zinc ionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Cytosol; Extracellular exosome

Involvement in disease

Fructosuria (FRUCT):
Benign defect of intermediary metabolism.

Helsley, R. N., Park, S. H., Vekaria, H. J., Sullivan, P. G., Conroy, L. R., Sun, R. C., ... & Softic, S. (2023). Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation. Journal of Hepatology, 79(1), 25-42.

Tee, S. S., Kim, N., Cullen, Q., Eskandari, R., Mamakhanyan, A., Srouji, R. M., ... & Keshari, K. R. (2022). Ketohexokinase-mediated fructose metabolism is lost in hepatocellular carcinoma and can be leveraged for metabolic imaging. Science Advances, 8(14), eabm7985.

Shepherd, E. L., Saborano, R., Northall, E., Matsuda, K., Ogino, H., Yashiro, H., ... & Lalor, P. F. (2021). Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis. JHEP Reports, 3(2), 100217.

Kim, J., Kang, J., Kang, Y. L., Woo, J., Kim, Y., Huh, J., & Park, J. W. (2020). Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 11(1), 5436.

Futatsugi, K., Smith, A. C., Tu, M., Raymer, B., Ahn, K., Coffey, S. B., ... & Magee, T. V. (2020). Discovery of PF-06835919: a potent inhibitor of ketohexokinase (KHK) for the treatment of metabolic disorders driven by the overconsumption of fructose. Journal of Medicinal Chemistry, 63(22), 13546-13560.

Hayasaki, T., Ishimoto, T., Doke, T., Hirayama, A., Soga, T., Furuhashi, K., ... & Kadomatsu, K. (2019). Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase. The Journal of Nutritional Biochemistry, 71, 54-62.

Lanaspa, M. A., Andres-Hernando, A., Orlicky, D. J., Cicerchi, C., Jang, C., Li, N., ... & Tolan, D. R. (2018). Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. The Journal of clinical investigation, 128(6), 2226-2238.

Gao, W., Li, N., Li, Z., Xu, J., & Su, C. (2018). Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma. Biochemical and biophysical research communications, 503(3), 1298-1306.

Miller, C. O., Yang, X., Lu, K., Cao, J., Herath, K., Rosahl, T. W., ... & Akiyama, T. E. (2018). Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects. American Journal of Physiology-Endocrinology and Metabolism, 315(3), E386-E393.

Liu, D., Sterpka, J. A., Vatner, D. F., Bell, M., Murray, S., Bhanot, S., ... & Samuel, V. (2018). Targeting ketohexokinase (KHK) with a novel antisense oligonucleotide (ASO) decreases de novo lipogenesis and improves insulin-mediated whole body glucose metabolism. Diabetes, 67(Supplement_1).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

KHK Matched Antibody Pair (658) (CAT#: APMAB-658LY)

KHK Matched Antibody Pair (659) (CAT#: APMAB-659LY)

KHK Matched Antibody Pair (660) (CAT#: APMAB-660LY)

Antibody Pairs

KHK Matched Antibody Pair (658) (CAT#: APMAB-658LY)

KHK Matched Antibody Pair (659) (CAT#: APMAB-659LY)

KHK Matched Antibody Pair (660) (CAT#: APMAB-660LY)

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Mouse Anti-KHK Recombinant Antibody (2B1) (CAT#: CBMAB-K0808-LY)

Mouse Anti-KHK Recombinant Antibody (3D1) (CAT#: CBMAB-K0811-LY)

Rabbit Anti-KHK Recombinant Antibody (CBLY1-075) (CAT#: CBMAB-K0804-LY)

Mouse Anti-KHK Recombinant Antibody (1F1) (CAT#: CBMAB-K0806-LY)

KHK Matched Antibody Pair (660) (CAT#: APMAB-660LY)

KHK Matched Antibody Pair (658) (CAT#: APMAB-658LY)

Mouse Anti-KHK Recombinant Antibody (4E9) (CAT#: CBMAB-K0813-LY)

Mouse Anti-KHK Recombinant Antibody (1D8) (CAT#: CBMAB-K0805-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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