Mouse Anti-KLHL2 Recombinant Antibody (8B8) (CBMAB-K1188-LY)

Name: Mouse Anti-KLHL2 Recombinant Antibody (8B8)
SKU: CBMAB-K1188-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8B8

Application

FC, IF, WB

Immunogen

KLHL2 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cells, as the immunogen

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.5 mg-1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

kelch-like 2, Mayven (Drosophila)

Introduction

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors.

Entrez Gene ID

11275

UniProt ID

O95198

Alternative Names

Kelch Like Family Member 2; Actin-Binding Protein Mayven; Mayven; Kelch (Drosophila)-Like 2 (Mayven); Kelch-Like 2; Mayven (Drosophila); Kelch-Like 2; Mayven; Kelch-Like Protein 2; ABP-KELCH; MAV;

Function

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation (By similarity).
Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors.

Biological Process

Protein ubiquitinationISS:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton; Cell projection; Cytoplasm, cytosol. A proportion colocalizes with the actin cytoskeleton. When over-expressed, colocalizes with NPTXR in perinuclear aggresomes (By similarity).

Zhang, E., Dong, X., Chen, S., Shao, J., Zhang, P., Wang, Y., & Wang, X. (2020). Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression. American Journal of Cancer Research, 10(10), 3345.

Zhang, H., Huang, H., Feng, X., Song, H., Zhang, Z., Shen, A., & Qiu, X. (2020). Deubiquitinase USP28 inhibits ubiquitin ligase KLHL2-mediated uridine-cytidine kinase 1 degradation and confers sensitivity to 5'-azacytidine-resistant human leukemia cells. Theranostics, 10(3), 1046.

Rodríguez, O. G., Pan, S. S., Patrice, P., Adams, L. D., Welch, J., Laux, R., ... & Scott, W. (2018). Common Variants in KLHL2 and C4orf50 Are Associated with Poorer Anti-VEGF Treatment Response in Age-Related Macular Degeneration. Investigative Ophthalmology & Visual Science, 59(9), 1425-1425.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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