Human Recombinant KLHL2 protein, GST Tag (V2LY-0526-LY5185)

Name: Human Recombinant KLHL2 protein, GST Tag
SKU: V2LY-0526-LY5185
Rating: 5 (1 reviews)

See all KLHL2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant KLHL2 protein, GST Tag consist of Amino Acid: 1-306 and predicts a molecular mass of 62.2 kDa.

Molecule Mass

62.2 kDa

Sequence

Amino Acid: 1-306

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

kelch-like 2, Mayven (Drosophila)

Function

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation (By similarity).
Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors.

Biological Process

Protein ubiquitinationISS:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton; Cell projection; Cytoplasm, cytosol. A proportion colocalizes with the actin cytoskeleton. When over-expressed, colocalizes with NPTXR in perinuclear aggresomes (By similarity).

Zhang, E., Dong, X., Chen, S., Shao, J., Zhang, P., Wang, Y., & Wang, X. (2020). Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression. American Journal of Cancer Research, 10(10), 3345.

Zhang, H., Huang, H., Feng, X., Song, H., Zhang, Z., Shen, A., & Qiu, X. (2020). Deubiquitinase USP28 inhibits ubiquitin ligase KLHL2-mediated uridine-cytidine kinase 1 degradation and confers sensitivity to 5'-azacytidine-resistant human leukemia cells. Theranostics, 10(3), 1046.

Rodríguez, O. G., Pan, S. S., Patrice, P., Adams, L. D., Welch, J., Laux, R., ... & Scott, W. (2018). Common Variants in KLHL2 and C4orf50 Are Associated with Poorer Anti-VEGF Treatment Response in Age-Related Macular Degeneration. Investigative Ophthalmology & Visual Science, 59(9), 1425-1425.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant KLHL2 protein, GST Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KLHL2 Recombinant Antibody (1G7) (CAT#: CBMAB-K1184-LY)

Mouse Anti-KLHL2 Recombinant Antibody (8B8) (CAT#: CBMAB-K1188-LY)

Mouse Anti-KLHL2 Recombinant Antibody (3H9) (CAT#: CBMAB-K1186-LY)

Mouse Anti-KLHL2 Recombinant Antibody (8D8) (CAT#: CBMAB-K1189-LY)

Mouse Anti-KLHL2 Recombinant Antibody (3G3) (CAT#: CBMAB-K1185-LY)

Mouse Anti-KLHL2 Recombinant Antibody (6D8) (CAT#: CBMAB-K1187-LY)

Mouse Anti-KLHL2 Recombinant Antibody (10C12) (CAT#: CBMAB-K1183-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry