Mouse Anti-KLHL25 Recombinant Antibody (CBT3978) (V2LY-0625-LY2468)

Name: Mouse Anti-KLHL25 Recombinant Antibody (CBT3978)
SKU: V2LY-0625-LY2468
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-KLHL25 Recombinant Antibody (CBT3978) (V2LY-0625-LY2468) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3978

Application

Immunogen

Purified recombinant fragment of human KLHL25 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kelch Like Family Member 25

Entrez Gene ID

64410

UniProt ID

Q9H0H3

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIAC F4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIAC F4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIAC F4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.

Biological Process

Protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cul3-RING ubiquitin ligase complex: Cytoplasm; Cytosol

Tian, M., Hao, F., Jin, X., Sun, X., Jiang, Y., Wang, Y., ... & Wei, M. (2021). ACLY ubiquitination by CUL3-KLHL25 induces the reprogramming of fatty acid metabolism to facilitate iTreg differentiation. Elife, 10, e62394.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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