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Rat Anti-Lefty1 Recombinant Antibody (CBYJL-2582) (CBMAB-L3017-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Left Right Determination Factor 1 (Lefty1). The antibody can be used for immunoassay techniques, such as WB.
See all Lefty1 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJL-2582
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lefty-1, Leu136-Pro368, Accession # Q64280.1.
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 136-368

Target

Full Name
LEFTY1
Introduction
Lefty1 encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which functions in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning.
Entrez Gene ID
13590
UniProt ID
Q64280
Alternative Names
Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052; left-right determination factor 1; TGF-beta-4; left-right determination, factor B; protein lefty-1; stimulated by retinoic acid gene 3 protein; transforming growth factor beta-4
Function
Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
Biological Process
BMP signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Determination of left/right symmetryISS:BHF-UCL
Heart morphogenesisISS:BHF-UCL
Negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Positive regulation of pathway-restricted SMAD protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
SMAD protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Secreted
PTM
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.

Ashiq, S., & Sabar, M. F. (2023). Association of maternal hypertension and diabetes with variants of the NKX2-5, LEFTY1 and LEFTY2 genes in children with congenital heart defects: a case-control study from Pakistani Population. Molecular Biology Reports, 1-8.

Zhao, Y., Yu, Y., Wang, S., Li, J., & Teng, L. (2022). Small extracellular vesicles encapsulating lefty1 mRNA inhibit hepatic fibrosis. Asian Journal of Pharmaceutical Sciences, 17(5), 630-640.

Li, C. Y., Zhang, J. R., Li, X. X., Zhao, L., Xi, H., Hu, W. N., & Li, S. N. (2021). Lefty1 ameliorates post-infarction fibrosis by suppressing p-Smad2 and p-ERK1/2 signaling pathways. Journal of cardiovascular translational research, 14, 636-646.

Skok, D. J., Hauptman, N., Jerala, M., & Zidar, N. (2021). Expression of Cytokine-Coding Genes BMP8B, LEFTY1 and INSL5 Could Distinguish between Ulcerative Colitis and Crohn’s Disease. Genes, 12(10), 1477.

Zabala, M., Lobo, N. A., Antony, J., Heitink, L. S., Gulati, G. S., Lam, J., ... & Clarke, M. F. (2020). LEFTY1 is a dual-SMAD inhibitor that promotes mammary progenitor growth and tumorigenesis. Cell Stem Cell, 27(2), 284-299.

Zhang, L., Liu, X., Liang, J., Wu, J., Tan, D., & Hu, W. (2020). Lefty-1 inhibits renal epithelial–mesenchymal transition by antagonizing the TGF-β/Smad signaling pathway. Journal of Molecular Histology, 51(1), 77-87.

Zhang, L., Xu, C., Hu, W., Wu, P., Qin, C., & Zhang, J. (2018). Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway. International journal of molecular medicine, 41(3), 1293-1304.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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