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Mouse Anti-LIG1 Recombinant Antibody (5H5) (CBMAB-D2105-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DNA Ligase 1 (LIG1). The antibody can be used for immunoassay techniques, such as FC, ICC, IF, IHC-P, IP, WB.
See all LIG1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
5H5
Antibody Isotype
IgG1
Application
FC, ICC, IF, IHC-P, IP, WB

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Ligase 1
Introduction
LIG1 is a member of the ATP-dependent DNA ligase protein family. LIG1 functions in DNA replication, recombination, and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers.
Entrez Gene ID
Human3978
Mouse16881
Rat81513
UniProt ID
HumanQ96JA1
MouseP37913
RatQ9JHY8
Alternative Names
DNA Ligase 1
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Base-excision repairManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIBA:GO_Central
DNA recombinationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mismatch repairTAS:Reactome
Okazaki fragment processing involved in mitotic DNA replicationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus

Sallmyr, A., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2023). Mammalian DNA ligases; roles in maintaining genome integrity. Journal of Molecular Biology, 168276.

Silva, J. M., Jones, A., Sibson, K., Bibi, S., Jeggo, P., Woodbine, L., ... & Amrolia, P. J. (2021). Haematopoietic stem cell transplantation for DNA ligase 1 deficiency. Journal of Clinical Immunology, 41, 238-242.

Jurkiw, T. J., Tumbale, P. P., Schellenberg, M. J., Cunningham-Rundles, C., Williams, R. S., & O’Brien, P. J. (2021). LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency. Nucleic acids research, 49(3), 1619-1630.

Sallmyr, A., Rashid, I., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2020). Human DNA ligases in replication and repair. DNA repair, 93, 102908.

Tang, Q., Kamble, P., & Çağlayan, M. (2020). DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. Mutagenesis, 35(5), 391-404.

Tomkinson, A. E., Naila, T., & Khattri Bhandari, S. (2020). Altered DNA ligase activity in human disease. Mutagenesis, 35(1), 51-60.

Liddiard, K., Ruis, B., Kan, Y., Cleal, K., Ashelford, K. E., Hendrickson, E. A., & Baird, D. M. (2019). DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase. Nucleic acids research, 47(5), 2402-2424.

Maffucci, P., Chavez, J., Jurkiw, T. J., O’Brien, P. J., Abbott, J. K., Reynolds, P. R., ... & Cunningham-Rundles, C. (2018). Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. The Journal of clinical investigation, 128(12), 5489-5504.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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