Rabbit Anti-LIG1 Recombinant Antibody (
7B2) (V2LY-0725-LY1425)

Name: Rabbit Anti-LIG1 Recombinant Antibody (7B2)
SKU: V2LY-0725-LY1425
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-LIG1 Recombinant Antibody (7B2) (V2LY-0725-LY1425) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: DNA Ligase I antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 102, 89, 99 kDa
Observed band size: 140 kDa

Rabbit Anti-LIG1 Recombinant Antibody (7B2) (V2LY-0725-LY1425) in IHC

IHC image of V2LY-0725-LY1425 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-LIG1 Recombinant Antibody (7B2) (V2LY-0725-LY1425) in IHC

IHC image of V2LY-0725-LY1425 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7B2

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human DNA Ligase I.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Ligase 1

Entrez Gene ID

3978

UniProt ID

P18858

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Biological Process

Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Base-excision repairManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIBA:GO_Central
DNA recombinationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mismatch repairTAS:Reactome
Okazaki fragment processing involved in mitotic DNA replicationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Sallmyr, A., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2023). Mammalian DNA ligases; roles in maintaining genome integrity. Journal of Molecular Biology, 168276.

Silva, J. M., Jones, A., Sibson, K., Bibi, S., Jeggo, P., Woodbine, L., ... & Amrolia, P. J. (2021). Haematopoietic stem cell transplantation for DNA ligase 1 deficiency. Journal of Clinical Immunology, 41, 238-242.

Jurkiw, T. J., Tumbale, P. P., Schellenberg, M. J., Cunningham-Rundles, C., Williams, R. S., & O’Brien, P. J. (2021). LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency. Nucleic acids research, 49(3), 1619-1630.

Sallmyr, A., Rashid, I., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2020). Human DNA ligases in replication and repair. DNA repair, 93, 102908.

Tang, Q., Kamble, P., & Çağlayan, M. (2020). DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. Mutagenesis, 35(5), 391-404.

Tomkinson, A. E., Naila, T., & Khattri Bhandari, S. (2020). Altered DNA ligase activity in human disease. Mutagenesis, 35(1), 51-60.

Liddiard, K., Ruis, B., Kan, Y., Cleal, K., Ashelford, K. E., Hendrickson, E. A., & Baird, D. M. (2019). DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase. Nucleic acids research, 47(5), 2402-2424.

Maffucci, P., Chavez, J., Jurkiw, T. J., O’Brien, P. J., Abbott, J. K., Reynolds, P. R., ... & Cunningham-Rundles, C. (2018). Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. The Journal of clinical investigation, 128(12), 5489-5504.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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