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Mouse Anti-LIG1 Recombinant Antibody (CBYCL-315) (CBMAB-L0216-YC)

Provided herein is a Mouse monoclonal antibody against Human LIG1. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all LIG1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYCL-315
Antibody Isotype
IgG2a
Application
ELISA, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4, with 0.09% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 810-920

Target

Full Name
DNA Ligase 1
Introduction
LIG1 is a member of the ATP-dependent DNA ligase protein family. LIG1 functions in DNA replication, recombination, and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers.
Entrez Gene ID
3978
UniProt ID
P18858
Alternative Names
LigI; AL033288
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Base-excision repairManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIBA:GO_Central
DNA recombinationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mismatch repairTAS:Reactome
Okazaki fragment processing involved in mitotic DNA replicationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus

Sallmyr, A., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2023). Mammalian DNA ligases; roles in maintaining genome integrity. Journal of Molecular Biology, 168276.

Silva, J. M., Jones, A., Sibson, K., Bibi, S., Jeggo, P., Woodbine, L., ... & Amrolia, P. J. (2021). Haematopoietic stem cell transplantation for DNA ligase 1 deficiency. Journal of Clinical Immunology, 41, 238-242.

Jurkiw, T. J., Tumbale, P. P., Schellenberg, M. J., Cunningham-Rundles, C., Williams, R. S., & O’Brien, P. J. (2021). LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency. Nucleic acids research, 49(3), 1619-1630.

Sallmyr, A., Rashid, I., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2020). Human DNA ligases in replication and repair. DNA repair, 93, 102908.

Tang, Q., Kamble, P., & Çağlayan, M. (2020). DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. Mutagenesis, 35(5), 391-404.

Tomkinson, A. E., Naila, T., & Khattri Bhandari, S. (2020). Altered DNA ligase activity in human disease. Mutagenesis, 35(1), 51-60.

Liddiard, K., Ruis, B., Kan, Y., Cleal, K., Ashelford, K. E., Hendrickson, E. A., & Baird, D. M. (2019). DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase. Nucleic acids research, 47(5), 2402-2424.

Maffucci, P., Chavez, J., Jurkiw, T. J., O’Brien, P. J., Abbott, J. K., Reynolds, P. R., ... & Cunningham-Rundles, C. (2018). Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. The Journal of clinical investigation, 128(12), 5489-5504.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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