Mouse Anti-LMAN1 Recombinant Antibody (CBYJL-1799) (CBMAB-L1739-YJ)

Name: Mouse Anti-LMAN1 Recombinant Antibody (CBYJL-1799)
SKU: CBMAB-L1739-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1799

Application

Immunogen

Full length human recombinant protein of human LMAN1(NP_005561) produced in Hek293T cell.ImmunogenType: Recombinant protein.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

lectin, mannose-binding, 1

Introduction

LMAN1 is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. LMAN1 has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. Diseases associated with LMAN1 include Factor V And Factor Viii, Combined Deficiency Of, 1 and Factor V And Factor Viii, Combined Deficiency Of, 2. Among its related pathways are Metabolism of proteins and Transport to the Golgi and subsequent modification. Gene Ontology (GO) annotations related to this gene include unfolded protein binding and mannose binding. An important paralog of this gene is LMAN1L.Mannose-specific lectin. LMAN1 may recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

Entrez Gene ID

3998

UniProt ID

P49257

Alternative Names

MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53

Function

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

Biological Process

Blood coagulationManual Assertion Based On ExperimentTAS:ProtInc
Endoplasmic reticulum organizationManual Assertion Based On ExperimentIBA:GO_Central
Endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central
Golgi organizationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of protein targeting to mitochondrionManual Assertion Based On ExperimentHMP:ParkinsonsUK-UCL
Positive regulation of organelle organizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein foldingManual Assertion Based On ExperimentTAS:ProtInc
Protein transportIEA:UniProtKB-KW

Cellular Location

Endoplasmic reticulum-Golgi intermediate compartment membrane
Golgi apparatus membrane
Endoplasmic reticulum membrane

Involvement in disease

Factor V and factor VIII combined deficiency 1 (F5F8D1):
A blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.

Topology

Lumenal: 31-477
Helical: 478-498
Cytoplasmic: 499-510

PTM

The N-terminal may be partly blocked.

Miller, M. H., Swaby, L. G., Vailoces, V. S., LaFratta, M., Zhang, Y., Zhu, X., ... & Tigno-Aranjuez, J. T. (2023). LMAN1 is a receptor for house dust mite allergens. Cell reports, 42(3).

Zhang, Y., Liu, Z., & Zhang, B. (2023). Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII. Blood Advances, 7(7), 1286-1296.

Tang, V. T., Abbineni, P. S., da Veiga Leprevost, F., Basrur, V., Emmer, B. T., Nesvizhskii, A., & Ginsburg, D. (2023). Identification of LMAN1 and SURF4 dependent secretory cargoes. bioRxiv, 2023-04.

Zhang, Y., Zhu, M., Zheng, C., Wei, W., Emmer, B. T., & Zhang, B. (2022). LMAN1–MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin. Biochemical Journal, 479(7), 839-855.

Du, Q., Lin, Y., Zhang, W., He, F., Xu, Y., & Chen, Z. (2022). Bioinformatics analysis of LMAN1 expression, clinical characteristics, and its effects on cell proliferation and invasion in glioma. Brain Research, 1789, 147952.

Alsheikh, S., Alghamdi, R., Alqatari, A., Alfareed, A., & AlSaleh, M. (2022). Combined factor V and VIII deficiency with LMAN1 mutation: a report of 3 Saudi siblings. The American Journal of Case Reports, 23, e937312-1.

Zhang, Y., Liu, Z., & Zhang, B. (2022). Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of factor V and factor VIII. bioRxiv, 2022-09.

Everett, L. A., Khoriaty, R. N., Zhang, B., & Ginsburg, D. (2020). Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression. Blood Advances, 4(22), 5635-5643.

Fu, Y. L., Zhang, B., & Mu, T. W. (2019). LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochemical and biophysical research communications, 511(2), 356-362.

Zhu, M., Zheng, C., Wei, W., Everett, L., Ginsburg, D., & Zhang, B. (2018). Analysis of MCFD2-and LMAN1-deficient mice demonstrates distinct functions in vivo. Blood Advances, 2(9), 1014-1021.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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