Mouse Anti-MAGEA4 Recombinant Antibody (
1G10H5) (V2LY-0725-LY165)

Name: Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5)
SKU: V2LY-0725-LY165
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5) (V2LY-0725-LY165) in IHC

IHC image of V2LY-0725-LY165 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5) (V2LY-0725-LY165) in IHC

IHC image of V2LY-0725-LY165 diluted at 1:200 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5) (V2LY-0725-LY165) in IHC

IHC image of V2LY-0725-LY165 diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5) (V2LY-0725-LY165) in IHC

IHC image of V2LY-0725-LY165 diluted at 1:200 and staining in paraffin-embedded human gastric cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-MAGEA4 Recombinant Antibody (1G10H5) (V2LY-0725-LY165) in FC

Overlay Peak curve showing A375 cells stained with the target protein (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1G10H5

Application

ELISA, IHC, FC

Immunogen

Recombinant Human Melanoma-associated antigen 4 protein (1-317AA).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:200-1:500
IHC	1:200-1:500
FC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MAGE Family Member A4

Entrez Gene ID

4103

UniProt ID

P43358

Function

Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486).
Also negatively regulates p53-mediated apoptosis (PubMed:22842486).

Biological Process

Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus

Freiberger, S. N., Holzmann, D., Morand, G. B., Hüllner, M., Levesque, M. P., Dummer, R., ... & Rupp, N. J. (2023). Combinational expression of tumor testis antigens NY-ESO-1, MAGE-A3, and MAGE-A4 predicts response to immunotherapy in mucosal melanoma patients. Journal of Cancer Research and Clinical Oncology, 149(9), 5645-5653.

Connacher, R., Avraam, K., Ross, E., Schneck, M., Van Kerckhoven, M., Van Rossom, S., ... & Wang, T. (2023). 198 Development, validation and concordance of two MAGE-A4 immunohistochemistry (IHC) assays to establish prognostic value of MAGE-A4 expression in synovial sarcoma.

Fleming, M. C., Chiou, L. F., Tumbale, P. P., Droby, G. N., Lim, J., Norris-Drouin, J. L., ... & Bowers, A. A. (2022). Discovery and structural basis of the selectivity of potent cyclic peptide inhibitors of MAGE-A4. Journal of medicinal chemistry, 65(10), 7231-7245.

Hashimoto, K., Nishimura, S., Ito, T., Oka, N., Kakinoki, R., & Akagi, M. (2022). Clinicopathological assessment of cancer/testis antigens NY-ESO-1 and MAGE-A4 in osteosarcoma. European Journal of Histochemistry: EJH, 66(3).

Davari, K., Holland, T., Prassmayer, L., Longinotti, G., Ganley, K. P., Pechilis, L. J., ... & Ellinger, C. (2021). Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy. Journal for Immunotherapy of Cancer, 9(3).

Coles, C. H., McMurran, C., Lloyd, A., Hock, M., Hibbert, L., Raman, M. C., ... & Harper, S. (2020). T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4. Journal of Biological Chemistry, 295(33), 11486-11494.

Ishihara, M., Kageyama, S., Miyahara, Y., Ishikawa, T., Ueda, S., Soga, N., ... & Shiku, H. (2020). MAGE-A4, NY-ESO-1 and SAGE mRNA expression rates and co-expression relationships in solid tumours. BMC cancer, 20(1), 1-8.

Sanderson, J. P., Crowley, D. J., Wiedermann, G. E., Quinn, L. L., Crossland, K. L., Tunbridge, H. M., ... & Gerry, A. B. (2020). Preclinical evaluation of an affinity-enhanced MAGE-A4-specific T-cell receptor for adoptive T-cell therapy. Oncoimmunology, 9(1), 1682381.

Kakimoto, T., Matsumine, A., Kageyama, S., Asanuma, K., Matsubara, T., Nakamura, T., ... & Sudo, A. (2019). Immunohistochemical expression and clinicopathological assessment of the cancer testis antigens NY‑ESO‑1 and MAGE‑A4 in high‑grade soft‑tissue sarcoma. Oncology Letters, 17(4), 3937-3943.

Van Tine, B. A., Butler, M. O., Araujo, D., Johnson, M. L., Clarke, J., Liebner, D., ... & Hong, D. S. (2019). ADP-A2M4 (MAGE-A4) in patients with synovial sarcoma. Annals of Oncology, 30, v684-v685.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MAGEA4 Matched Antibody Pair (704) (CAT#: APMAB-704LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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