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Mouse Anti-MAGEA4 Recombinant Antibody (CBT2391) (V2LY-0625-LY1799)

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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2391
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
Purified recombinant fragment of human MAGEA4 (AA: 1-225) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MAGE Family Member A4
Entrez Gene ID
UniProt ID
Function
Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486).
Also negatively regulates p53-mediated apoptosis (PubMed:22842486).
Biological Process
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
More Infomation

Freiberger, S. N., Holzmann, D., Morand, G. B., Hüllner, M., Levesque, M. P., Dummer, R., ... & Rupp, N. J. (2023). Combinational expression of tumor testis antigens NY-ESO-1, MAGE-A3, and MAGE-A4 predicts response to immunotherapy in mucosal melanoma patients. Journal of Cancer Research and Clinical Oncology, 149(9), 5645-5653.

Connacher, R., Avraam, K., Ross, E., Schneck, M., Van Kerckhoven, M., Van Rossom, S., ... & Wang, T. (2023). 198 Development, validation and concordance of two MAGE-A4 immunohistochemistry (IHC) assays to establish prognostic value of MAGE-A4 expression in synovial sarcoma.

Fleming, M. C., Chiou, L. F., Tumbale, P. P., Droby, G. N., Lim, J., Norris-Drouin, J. L., ... & Bowers, A. A. (2022). Discovery and structural basis of the selectivity of potent cyclic peptide inhibitors of MAGE-A4. Journal of medicinal chemistry, 65(10), 7231-7245.

Hashimoto, K., Nishimura, S., Ito, T., Oka, N., Kakinoki, R., & Akagi, M. (2022). Clinicopathological assessment of cancer/testis antigens NY-ESO-1 and MAGE-A4 in osteosarcoma. European Journal of Histochemistry: EJH, 66(3).

Davari, K., Holland, T., Prassmayer, L., Longinotti, G., Ganley, K. P., Pechilis, L. J., ... & Ellinger, C. (2021). Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy. Journal for Immunotherapy of Cancer, 9(3).

Coles, C. H., McMurran, C., Lloyd, A., Hock, M., Hibbert, L., Raman, M. C., ... & Harper, S. (2020). T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4. Journal of Biological Chemistry, 295(33), 11486-11494.

Ishihara, M., Kageyama, S., Miyahara, Y., Ishikawa, T., Ueda, S., Soga, N., ... & Shiku, H. (2020). MAGE-A4, NY-ESO-1 and SAGE mRNA expression rates and co-expression relationships in solid tumours. BMC cancer, 20(1), 1-8.

Sanderson, J. P., Crowley, D. J., Wiedermann, G. E., Quinn, L. L., Crossland, K. L., Tunbridge, H. M., ... & Gerry, A. B. (2020). Preclinical evaluation of an affinity-enhanced MAGE-A4-specific T-cell receptor for adoptive T-cell therapy. Oncoimmunology, 9(1), 1682381.

Kakimoto, T., Matsumine, A., Kageyama, S., Asanuma, K., Matsubara, T., Nakamura, T., ... & Sudo, A. (2019). Immunohistochemical expression and clinicopathological assessment of the cancer testis antigens NY‑ESO‑1 and MAGE‑A4 in high‑grade soft‑tissue sarcoma. Oncology Letters, 17(4), 3937-3943.

Van Tine, B. A., Butler, M. O., Araujo, D., Johnson, M. L., Clarke, J., Liebner, D., ... & Hong, D. S. (2019). ADP-A2M4 (MAGE-A4) in patients with synovial sarcoma. Annals of Oncology, 30, v684-v685.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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