Human Recombinant MAGEA4 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY5418)

Name: Human Recombinant MAGEA4 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY5418
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant MAGEA4 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-317 and predicts a molecular mass of 37.54 kDa.

Molecule Mass

37.54 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-317

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MAGE Family Member A4

Function

Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486).
Also negatively regulates p53-mediated apoptosis (PubMed:22842486).

Biological Process

Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus

Freiberger, S. N., Holzmann, D., Morand, G. B., Hüllner, M., Levesque, M. P., Dummer, R., ... & Rupp, N. J. (2023). Combinational expression of tumor testis antigens NY-ESO-1, MAGE-A3, and MAGE-A4 predicts response to immunotherapy in mucosal melanoma patients. Journal of Cancer Research and Clinical Oncology, 149(9), 5645-5653.

Connacher, R., Avraam, K., Ross, E., Schneck, M., Van Kerckhoven, M., Van Rossom, S., ... & Wang, T. (2023). 198 Development, validation and concordance of two MAGE-A4 immunohistochemistry (IHC) assays to establish prognostic value of MAGE-A4 expression in synovial sarcoma.

Fleming, M. C., Chiou, L. F., Tumbale, P. P., Droby, G. N., Lim, J., Norris-Drouin, J. L., ... & Bowers, A. A. (2022). Discovery and structural basis of the selectivity of potent cyclic peptide inhibitors of MAGE-A4. Journal of medicinal chemistry, 65(10), 7231-7245.

Hashimoto, K., Nishimura, S., Ito, T., Oka, N., Kakinoki, R., & Akagi, M. (2022). Clinicopathological assessment of cancer/testis antigens NY-ESO-1 and MAGE-A4 in osteosarcoma. European Journal of Histochemistry: EJH, 66(3).

Davari, K., Holland, T., Prassmayer, L., Longinotti, G., Ganley, K. P., Pechilis, L. J., ... & Ellinger, C. (2021). Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy. Journal for Immunotherapy of Cancer, 9(3).

Coles, C. H., McMurran, C., Lloyd, A., Hock, M., Hibbert, L., Raman, M. C., ... & Harper, S. (2020). T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4. Journal of Biological Chemistry, 295(33), 11486-11494.

Ishihara, M., Kageyama, S., Miyahara, Y., Ishikawa, T., Ueda, S., Soga, N., ... & Shiku, H. (2020). MAGE-A4, NY-ESO-1 and SAGE mRNA expression rates and co-expression relationships in solid tumours. BMC cancer, 20(1), 1-8.

Sanderson, J. P., Crowley, D. J., Wiedermann, G. E., Quinn, L. L., Crossland, K. L., Tunbridge, H. M., ... & Gerry, A. B. (2020). Preclinical evaluation of an affinity-enhanced MAGE-A4-specific T-cell receptor for adoptive T-cell therapy. Oncoimmunology, 9(1), 1682381.

Kakimoto, T., Matsumine, A., Kageyama, S., Asanuma, K., Matsubara, T., Nakamura, T., ... & Sudo, A. (2019). Immunohistochemical expression and clinicopathological assessment of the cancer testis antigens NY‑ESO‑1 and MAGE‑A4 in high‑grade soft‑tissue sarcoma. Oncology Letters, 17(4), 3937-3943.

Van Tine, B. A., Butler, M. O., Araujo, D., Johnson, M. L., Clarke, J., Liebner, D., ... & Hong, D. S. (2019). ADP-A2M4 (MAGE-A4) in patients with synovial sarcoma. Annals of Oncology, 30, v684-v685.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

MAGEA4 Matched Antibody Pair (704) (CAT#: APMAB-704LY)

Antibody Pairs

MAGEA4 Matched Antibody Pair (704) (CAT#: APMAB-704LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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