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Mouse Anti-MGRN1 Recombinant Antibody (3C3) (CBMAB-R0584-CN)

This product is a Mouse antibody that recognizes MGRN1. The antibody 3C3 can be used for immunoassay techniques such as: WB, FC, IF.
See all MGRN1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Monkey
Clone
3C3
Antibody Isotype
IgG1
Application
WB, FC, IF

Basic Information

Specificity
Human, Monkey
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
Mahogunin Ring Finger 1
Introduction
Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro. [supplied by OMIM, Apr 2004]
Entrez Gene ID
Human23295
Monkey709272
UniProt ID
HumanO60291
MonkeyF7FXG6
Alternative Names
Mahogunin Ring Finger 1; Mahogunin Ring Finger 1, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase MGRN1; Mahogunin RING Finger Protein 1; RING Finger Protein 156; RNF156; Probable E3 Ubiquitin-Protein Ligase MGRN1;
Function
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).
Biological Process
Endosome to lysosome transport Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: UniProtKB
Protein monoubiquitination Source: UniProtKB
Protein polyubiquitination Source: Ensembl
Protein ubiquitination Source: GO_Central
Cellular Location
Endosome
Early endosome
Note: The endosomal localization is dependent on the interaction with TSG101.
Isoform 1:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 2:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 3:
Plasma membrane
Cell membrane
cytosol
Isoform 4:
Plasma membrane
Cell membrane
cytosol
PTM
Autoubiquitinated in vitro.

Cerdido, S., Abrisqueta, M., Sánchez-Beltrán, J., Lambertos, A., Castejón-Griñán, M., Muñoz, C., ... & Herraiz, C. (2024). MGRN1 depletion promotes intercellular adhesion in melanoma by upregulation of E-cadherin and inhibition of CDC42. Cancer Letters, 581, 216484.

Abrisqueta, M., Cerdido, S., Sánchez-Beltrán, J., Martínez-Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life, 12(8), 1118.

Abrisqueta Gonzalez, M., Cerdido, S., Sánchez Beltrán, J., Martínez Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker.

Li, X. F., Sun, H. Y., Hua, T., Zhang, H. B., Tian, Y. J., Li, Y., & Kang, S. (2021). Promoter methylation of the MGRN1 gene predicts prognosis and response to chemotherapy of high-grade serous ovarian cancer patients. Frontiers in Oncology, 11, 659254.

Li, X., Sun, H., Tian, H., Zhang, H., Tian, Y., Li, Y., & Kang, S. (2021). Promoter Methylation of the MGRN1 Gene Response to Chemotherapy of Epithelial Ovarian Cancer Patients.

Nawaratne, V., Kudumala, S., Kakad, P. P., & Godenschwege, T. A. (2021). The conserved MASRPF motif in the Attractin homolog, Distracted, is required for association with Drosophila E3-ligase Mgrn1. microPublication Biology, 2021.

Gunn, T. M., Silvius, D., Lester, A., & Gibbs, B. (2019). Chronic and age-dependent effects of the spongiform neurodegeneration-associated MGRN1 E3 ubiquitin ligase on mitochondrial homeostasis. Mammalian Genome, 30, 151-165.

Mukherjee, R., Bhattacharya, A., Sau, A., Basil, S., Chakrabarti, S., & Chakrabarti, O. (2019). Calmodulin regulates MGRN1‐GP78 interaction mediated ubiquitin proteasomal degradation system. The FASEB Journal, 33(2), 1927-1945.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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