Human Recombinant MGRN1, Active protein, GST Tag (V2LY-0526-LY5577)

Name: Human Recombinant MGRN1, Active protein, GST Tag
SKU: V2LY-0526-LY5577
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MGRN1, Active protein, GST Tag consist of Amino Acid: 2-end and predicts a molecular mass of 96 kDa.

Molecule Mass

96 kDa

Sequence

Amino Acid: 2-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Mahogunin Ring Finger 1

Function

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).

Biological Process

Endosome to lysosome transport Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: UniProtKB
Protein monoubiquitination Source: UniProtKB
Protein polyubiquitination Source: Ensembl
Protein ubiquitination Source: GO_Central

Cellular Location

Endosome
Early endosome
Note: The endosomal localization is dependent on the interaction with TSG101.
Isoform 1:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 2:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 3:
Plasma membrane
Cell membrane
cytosol
Isoform 4:
Plasma membrane
Cell membrane
cytosol

PTM

Autoubiquitinated in vitro.

Cerdido, S., Abrisqueta, M., Sánchez-Beltrán, J., Lambertos, A., Castejón-Griñán, M., Muñoz, C., ... & Herraiz, C. (2024). MGRN1 depletion promotes intercellular adhesion in melanoma by upregulation of E-cadherin and inhibition of CDC42. Cancer Letters, 581, 216484.

Abrisqueta, M., Cerdido, S., Sánchez-Beltrán, J., Martínez-Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life, 12(8), 1118.

Abrisqueta Gonzalez, M., Cerdido, S., Sánchez Beltrán, J., Martínez Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker.

Li, X. F., Sun, H. Y., Hua, T., Zhang, H. B., Tian, Y. J., Li, Y., & Kang, S. (2021). Promoter methylation of the MGRN1 gene predicts prognosis and response to chemotherapy of high-grade serous ovarian cancer patients. Frontiers in Oncology, 11, 659254.

Li, X., Sun, H., Tian, H., Zhang, H., Tian, Y., Li, Y., & Kang, S. (2021). Promoter Methylation of the MGRN1 Gene Response to Chemotherapy of Epithelial Ovarian Cancer Patients.

Nawaratne, V., Kudumala, S., Kakad, P. P., & Godenschwege, T. A. (2021). The conserved MASRPF motif in the Attractin homolog, Distracted, is required for association with Drosophila E3-ligase Mgrn1. microPublication Biology, 2021.

Gunn, T. M., Silvius, D., Lester, A., & Gibbs, B. (2019). Chronic and age-dependent effects of the spongiform neurodegeneration-associated MGRN1 E3 ubiquitin ligase on mitochondrial homeostasis. Mammalian Genome, 30, 151-165.

Mukherjee, R., Bhattacharya, A., Sau, A., Basil, S., Chakrabarti, S., & Chakrabarti, O. (2019). Calmodulin regulates MGRN1‐GP78 interaction mediated ubiquitin proteasomal degradation system. The FASEB Journal, 33(2), 1927-1945.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

MGRN1 Matched Antibody Pair (733) (CAT#: APMAB-733LY)

MGRN1 Matched Antibody Pair (734) (CAT#: APMAB-734LY)

Antibody Pairs

MGRN1 Matched Antibody Pair (733) (CAT#: APMAB-733LY)

MGRN1 Matched Antibody Pair (734) (CAT#: APMAB-734LY)

Related Products

MGRN1 Matched Antibody Pair (733) (CAT#: APMAB-733LY)

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Mouse Anti-MGRN1 Recombinant Antibody (CBFYM-0638) (CAT#: CBMAB-M0773-FY)

MGRN1 Matched Antibody Pair (734) (CAT#: APMAB-734LY)

Mouse Anti-MGRN1 Recombinant Antibody (CBCNR-587) (CAT#: CBMAB-R0585-CN)

Mouse Anti-MGRN1 (AA 477-576) Recombinant Antibody (CBFYM-2189) (CAT#: CBMAB-M2371-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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