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Mouse Anti-MGRN1 (AA 477-576) Recombinant Antibody (CBFYM-2189) (CBMAB-M2371-FY)

This product is mouse antibody that recognizes MGRN1. The antibody CBFYM-2189 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all MGRN1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2189
Antibody Isotype
IgG2a, k
Application
ELISA, IF, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: PPPLGGAELA LRESSSPESF ITEEVDESSS PQQGTRAASI ENVLQDSSPE HCGRGPPADI YLPGRPTSME TAHGLATTSP TWPPLGGPSP DPSAAELTPL
Specificity
Human
Antibody Isotype
IgG2a, k
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 477-576

Target

Full Name
Mahogunin Ring Finger 1
Introduction
Mahogunin is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.
Entrez Gene ID
23295
UniProt ID
O60291
Alternative Names
Mahogunin Ring Finger 1; Mahogunin Ring Finger 1, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase MGRN1; Mahogunin RING Finger Protein 1; RING Finger Protein 156; RNF156; Probable E3 Ubiquitin-Protein Ligase MGRN1
Function
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).
Biological Process
Endosome to lysosome transport Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: UniProtKB
Protein monoubiquitination Source: UniProtKB
Protein polyubiquitination Source: Ensembl
Protein ubiquitination Source: GO_Central
Cellular Location
Endosome
Early endosome
Note: The endosomal localization is dependent on the interaction with TSG101.
Isoform 1:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 2:
Nucleus
cytosol
Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
Isoform 3:
Plasma membrane
Cell membrane
cytosol
Isoform 4:
Plasma membrane
Cell membrane
cytosol
PTM
Autoubiquitinated in vitro.

Cerdido, S., Abrisqueta, M., Sánchez-Beltrán, J., Lambertos, A., Castejón-Griñán, M., Muñoz, C., ... & Herraiz, C. (2024). MGRN1 depletion promotes intercellular adhesion in melanoma by upregulation of E-cadherin and inhibition of CDC42. Cancer Letters, 581, 216484.

Abrisqueta, M., Cerdido, S., Sánchez-Beltrán, J., Martínez-Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life, 12(8), 1118.

Abrisqueta Gonzalez, M., Cerdido, S., Sánchez Beltrán, J., Martínez Vicente, I., Herraiz, C., Lambertos, A., ... & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker.

Li, X. F., Sun, H. Y., Hua, T., Zhang, H. B., Tian, Y. J., Li, Y., & Kang, S. (2021). Promoter methylation of the MGRN1 gene predicts prognosis and response to chemotherapy of high-grade serous ovarian cancer patients. Frontiers in Oncology, 11, 659254.

Li, X., Sun, H., Tian, H., Zhang, H., Tian, Y., Li, Y., & Kang, S. (2021). Promoter Methylation of the MGRN1 Gene Response to Chemotherapy of Epithelial Ovarian Cancer Patients.

Nawaratne, V., Kudumala, S., Kakad, P. P., & Godenschwege, T. A. (2021). The conserved MASRPF motif in the Attractin homolog, Distracted, is required for association with Drosophila E3-ligase Mgrn1. microPublication Biology, 2021.

Gunn, T. M., Silvius, D., Lester, A., & Gibbs, B. (2019). Chronic and age-dependent effects of the spongiform neurodegeneration-associated MGRN1 E3 ubiquitin ligase on mitochondrial homeostasis. Mammalian Genome, 30, 151-165.

Mukherjee, R., Bhattacharya, A., Sau, A., Basil, S., Chakrabarti, S., & Chakrabarti, O. (2019). Calmodulin regulates MGRN1‐GP78 interaction mediated ubiquitin proteasomal degradation system. The FASEB Journal, 33(2), 1927-1945.

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For research use only. Not intended for any clinical use.

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