Mouse Anti-MIB2 Recombinant Antibody (3A7) (CBMAB-A5480-LY)

Name: Mouse Anti-MIB2 Recombinant Antibody (3A7)
SKU: CBMAB-A5480-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A7

Application

sELISA, ELISA

Immunogen

MIB2 (AAH37542, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

mindbomb homolog 2 (Drosophila)

Entrez Gene ID

142678

UniProt ID

Q96AX9

Alternative Names

FLJ20648; FLJ39787; ZZANK1; ZZZ5

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.

Biological Process

Notch signaling pathway Source: UniProtKB-KW
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Protein ubiquitination Source: GO_Central

Cellular Location

Cytoplasm
Endosome
Note: Colocalizes with endosomal compartments.

PTM

Ubiquitinated. Possibly via autoubiquitination (By similarity).

Guo, Y., Chi, X., Sun, Y., Liu, J., Zhang, L., Cao, C., ... & Zhang, W. (2022). E3 ubiquitin-protein ligase 2 inhibits cell proliferation, migration, and invasion of non-small cell lung cancer through ubiquitination of Notch1. Acta Histochemica, 124(1), 151818.

Uematsu, A., Kido, K., Takahashi, H., Takahashi, C., Yanagihara, Y., Saeki, N., ... & Sawasaki, T. (2019). The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD. Journal of Biological Chemistry, 294(38), 14135-14148.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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