Mouse Anti-MIB2 (AA 418-520) Recombinant Antibody (CBFYM-2203) (CBMAB-M2386-FY)

Name: Mouse Anti-MIB2 (AA 418-520) Recombinant Antibody (CBFYM-2203)
SKU: CBMAB-M2386-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2203

Application

ICC, IF

Immunogen

Recombinant human Mind Bomb 2/MIB2 Ala418-Lys520

Specificity

Human

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 5% trehalose

Concentration

0.2 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 418-520

More Infomation

Target

Full Name

mindbomb homolog 2 (Drosophila)

Introduction

The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion.

Entrez Gene ID

142678

UniProt ID

Q96AX9

Alternative Names

Mindbomb E3 Ubiquitin Protein Ligase 2; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 1; Zinc Finger, ZZ Type With Ankyrin Repeat Domain 1; Putative NF-Kappa-B-Activating Protein 002N; RING-Type E3 Ubiquitin Transferase MIB2; Novel Zinc Finger Protein; Mind Bomb Homolog 2; Skeletrophin; Novelzin; ZZANK1

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.

Biological Process

Notch signaling pathway Source: UniProtKB-KW
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Protein ubiquitination Source: GO_Central

Cellular Location

Cytoplasm
Endosome
Note: Colocalizes with endosomal compartments.

PTM

Ubiquitinated. Possibly via autoubiquitination (By similarity).

Guo, Y., Chi, X., Sun, Y., Liu, J., Zhang, L., Cao, C., ... & Zhang, W. (2022). E3 ubiquitin-protein ligase 2 inhibits cell proliferation, migration, and invasion of non-small cell lung cancer through ubiquitination of Notch1. Acta Histochemica, 124(1), 151818.

Uematsu, A., Kido, K., Takahashi, H., Takahashi, C., Yanagihara, Y., Saeki, N., ... & Sawasaki, T. (2019). The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD. Journal of Biological Chemistry, 294(38), 14135-14148.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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