Mouse Anti-MYL9 Recombinant Antibody (3F2) (CBMAB-A5732-LY)

Name: Mouse Anti-MYL9 Recombinant Antibody (3F2)
SKU: CBMAB-A5732-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F2

Application

WB, ELISA

Immunogen

MYL9 (AAH02648.1, 1 a.a. ~ 118 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

myosin, light chain 9, regulatory

Introduction

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Entrez Gene ID

10398

UniProt ID

P24844

Alternative Names

LC20; MGC3505; MLC2; MRLC1; MYRL2

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655).

In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

Biological Process

Myofibril assembly Source: GO_Central
Platelet aggregation Source: UniProtKB
Regulation of muscle contraction Source: ProtInc

Cellular Location

Cytoskeleton
Other locations
cell cortex
Note: Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts.

Involvement in disease

Megacystis-microcolon-intestinal hypoperistalsis syndrome 4 (MMIHS4):
A form of megacystis-microcolon-intestinal hypoperistalsis syndrome, a congenital visceral myopathy primarily affecting females, and characterized by loss of smooth muscle contraction in the bladder and intestine. Affected individuals present at birth with functional obstruction of intestine, microcolon, dilation of bladder, and secondary hydronephrosis. The majority of cases have a fatal outcome due to malnutrition and sepsis, followed by multiorgan failure. MMIHS4 inheritance is autosomal recessive.

PTM

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation.

Kobayashi, H., Kimura, M. Y., Hasegawa, I., Suganuma, E., Ikehara, Y., Azuma, K., ... & Nakayama, T. (2023). Increased Myosin light chain 9 expression during Kawasaki disease vasculitis. Frontiers in Immunology, 13, 1036672.

Matsushita, K., Kobayashi, S., Akita, H., Konno, M., Asai, A., Noda, T., ... & Ishii, H. (2022). Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. Cancer Reports, 5(10), e1582.

Lv, M., Luo, L., & Chen, X. (2022). The landscape of prognostic and immunological role of myosin light chain 9 (MYL9) in human tumors. Immunity, Inflammation and Disease, 10(2), 241-254.

Feng, M., Dong, N., Zhou, X., Ma, L., & Xiang, R. (2022). Myosin light chain 9 promotes the proliferation, invasion, migration and angiogenesis of colorectal cancer cells by binding to Yes-associated protein 1 and regulating Hippo signaling. Bioengineered, 13(1), 96-106.

Yokoyama, M., Kimura, M. Y., Ito, T., Hayashizaki, K., Endo, Y., Wang, Y., ... & Nakayama, T. (2021). Myosin light chain 9/12 regulates the pathogenesis of inflammatory bowel disease. Frontiers in Immunology, 11, 594297.

Kruthika, B. S., Sugur, H., Nandaki, K., Arimappamagan, A., Paturu, K., & Santosh, V. (2019). Expression pattern and prognostic significance of myosin light chain 9 (MYL9): a novel biomarker in glioblastoma. Journal of Clinical Pathology.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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