Human Anti-MYL9 Recombinant Antibody (
6G11) (V2LY-0725-LY833)

Name: Human Anti-MYL9 Recombinant Antibody (6G11)
SKU: V2LY-0725-LY833
Rating: 5 (1 reviews)

See all MYL9 antibodies

Compare Online Inquiry

Tested Data

Human Anti-MYL9 Recombinant Antibody (6G11) (V2LY-0725-LY833) in ELISA

The Binding Activity of Human MYL9 with Anti-MYL9 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human MYL9 at 2 μg/mL can bind Anti-MYL9 recombinant antibody, the EC50 is 4.628-6.430 ng/mL.

Human Anti-MYL9 Recombinant Antibody (6G11) (V2LY-0725-LY833) in ELISA

The Binding Activity of Human MYL12B with Anti-MYL9 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human MYL12B at 2 μg/mL can bind Anti-MYL9 recombinant antibody, the EC50 is 7.760-8.646 ng/mL.

Human Anti-MYL9 Recombinant Antibody (6G11) (V2LY-0725-LY833) in ELISA

The Binding Activity of Human MYL12A with Anti-MYL9 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human MYL12A at 2 μg/mL can bind Anti-MYL9 recombinant antibody, the EC50 is 5.325-6.456 ng/mL.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Human

Clone

6G11

Application

ELISA

Immunogen

Recombinant Human MYL9 protein.

Host Species

Human

Specificity

Human

Antibody Isotype

IgG2

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

myosin, light chain 9, regulatory

Entrez Gene ID

10398

UniProt ID

P24844

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655).

In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

Biological Process

Myofibril assembly Source: GO_Central
Platelet aggregation Source: UniProtKB
Regulation of muscle contraction Source: ProtInc

Cellular Location

Cytoskeleton
Other locations
cell cortex
Note: Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts.

Involvement in disease

Megacystis-microcolon-intestinal hypoperistalsis syndrome 4 (MMIHS4):
A form of megacystis-microcolon-intestinal hypoperistalsis syndrome, a congenital visceral myopathy primarily affecting females, and characterized by loss of smooth muscle contraction in the bladder and intestine. Affected individuals present at birth with functional obstruction of intestine, microcolon, dilation of bladder, and secondary hydronephrosis. The majority of cases have a fatal outcome due to malnutrition and sepsis, followed by multiorgan failure. MMIHS4 inheritance is autosomal recessive.

PTM

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation.

Kobayashi, H., Kimura, M. Y., Hasegawa, I., Suganuma, E., Ikehara, Y., Azuma, K., ... & Nakayama, T. (2023). Increased Myosin light chain 9 expression during Kawasaki disease vasculitis. Frontiers in Immunology, 13, 1036672.

Matsushita, K., Kobayashi, S., Akita, H., Konno, M., Asai, A., Noda, T., ... & Ishii, H. (2022). Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. Cancer Reports, 5(10), e1582.

Lv, M., Luo, L., & Chen, X. (2022). The landscape of prognostic and immunological role of myosin light chain 9 (MYL9) in human tumors. Immunity, Inflammation and Disease, 10(2), 241-254.

Feng, M., Dong, N., Zhou, X., Ma, L., & Xiang, R. (2022). Myosin light chain 9 promotes the proliferation, invasion, migration and angiogenesis of colorectal cancer cells by binding to Yes-associated protein 1 and regulating Hippo signaling. Bioengineered, 13(1), 96-106.

Yokoyama, M., Kimura, M. Y., Ito, T., Hayashizaki, K., Endo, Y., Wang, Y., ... & Nakayama, T. (2021). Myosin light chain 9/12 regulates the pathogenesis of inflammatory bowel disease. Frontiers in Immunology, 11, 594297.

Kruthika, B. S., Sugur, H., Nandaki, K., Arimappamagan, A., Paturu, K., & Santosh, V. (2019). Expression pattern and prognostic significance of myosin light chain 9 (MYL9): a novel biomarker in glioblastoma. Journal of Clinical Pathology.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Anti-MYL9 Recombinant Antibody (
6G11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-MYL9 (AA 1-118) Recombinant Antibody (CBFYM-2968) (CAT#: CBMAB-M3163-FY)

Mouse Anti-MYL9 (Phosphorylated S19) Recombinant Antibody (4i15) (CAT#: PTM-CBMAB-0408LY)

Mouse Anti-MYL9 (Phosphorylated S20) Recombinant Antibody (CAT#: PTM-CBMAB-0978LY)

Mouse Anti-MYL9 Recombinant Antibody (CBFYM-2967) (CAT#: CBMAB-M3162-FY)

Mouse Anti-MYL9 Recombinant Antibody (3F2) (CAT#: CBMAB-A5732-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry