Mouse Anti-MYLIP Recombinant Antibody (3C10) (CBMAB-A5733-LY)

The product is antibody recognizes MYLIP. The antibody 3C10 immunoassay techniques such as: sELISA, ELISA.
See all MYLIP antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3C10

Antibody Isotype

IgG2a, κ

Application

sELISA, ELISA

Basic Information

Immunogen

MYLIP (AAH02860, 1 a.a. ~ 445 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

myosin regulatory light chain interacting protein

Introduction

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq]

Entrez Gene ID

29116

UniProt ID

Q8WY64

Alternative Names

MIR

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

Biological Process

Cholesterol homeostasis Source: Ensembl
Negative regulation of low-density lipoprotein particle clearance Source: Ensembl
Negative regulation of neuron projection development Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of protein catabolic process Source: Ensembl
Protein destabilization Source: Ensembl
Protein ubiquitination Source: UniProtKB
Regulation of low-density lipoprotein particle receptor catabolic process Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane

PTM

Autoubiquitinated.

References

Martins, I., Neves-Silva, D., Ascensão-Ferreira, M., Dias, A. F., Ribeiro, D., Isidro, A. F., ... & Saúde, L. (2023). Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players. International Journal of Molecular Sciences, 24(7), 6433.

Dong, Y. H., Zhou, C. J., Zhang, M. Y., Tao, J., Zhang, X. M., An, L., ... & Cang, M. (2021). MiR‐455‐5p monitors myotube morphogenesis by targeting mylip. Journal of Cellular Biochemistry, 122(3-4), 442-455.

Wang, H., Ma, L., Pan, X., Du, Z., & Chen, Y. (2021). Novel associations of SNPs MYLIP rs3757354 and ABCA1 2230806 gene with early-onset-preeclampsia: A case-control candidate genetic study. Pregnancy Hypertension, 23, 185-190.

Ni, M., Yan, Q., Xue, H., Du, Y., Zhao, S., & Zhao, Z. (2021). Identification of MYLIP gene and miRNA-802 involved in the growth and metastasis of cervical cancer cells. Cancer Biomarkers, 30(3), 287-298.

Wang, W., Li, F., Gan, P., Su, D., Li, G., Dang, L., & Peng, Y. (2021). The expression of myosin-regulated light chain interacting protein (MYLIP) in lung cancer and its inhibitory effects on lung carcinomas. Translational Cancer Research, 10(5), 2389.

Zhao, L., Zhou, Y., Zhao, Y., Li, Q., Zhou, J., & Mao, Y. (2020). Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY), 12(3), 2974.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-MYLIP (AA 127-385) Recombinant Antibody (CBFYM-2969)

Mouse Anti-MYLIP (AA 1-445) Recombinant Antibody (CBFYM-2970)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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