Human MYLIP ELISA Kit (V2LY-0626-LY4520)

Name: Human MYLIP ELISA Kit
SKU: V2LY-0626-LY4520
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0052 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

49.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

myosin regulatory light chain interacting protein

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

Biological Process

Cholesterol homeostasis Source: Ensembl
Negative regulation of low-density lipoprotein particle clearance Source: Ensembl
Negative regulation of neuron projection development Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of protein catabolic process Source: Ensembl
Protein destabilization Source: Ensembl
Protein ubiquitination Source: UniProtKB
Regulation of low-density lipoprotein particle receptor catabolic process Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane

PTM

Autoubiquitinated.

Martins, I., Neves-Silva, D., Ascensão-Ferreira, M., Dias, A. F., Ribeiro, D., Isidro, A. F., ... & Saúde, L. (2023). Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players. International Journal of Molecular Sciences, 24(7), 6433.

Dong, Y. H., Zhou, C. J., Zhang, M. Y., Tao, J., Zhang, X. M., An, L., ... & Cang, M. (2021). MiR‐455‐5p monitors myotube morphogenesis by targeting mylip. Journal of Cellular Biochemistry, 122(3-4), 442-455.

Wang, H., Ma, L., Pan, X., Du, Z., & Chen, Y. (2021). Novel associations of SNPs MYLIP rs3757354 and ABCA1 2230806 gene with early-onset-preeclampsia: A case-control candidate genetic study. Pregnancy Hypertension, 23, 185-190.

Ni, M., Yan, Q., Xue, H., Du, Y., Zhao, S., & Zhao, Z. (2021). Identification of MYLIP gene and miRNA-802 involved in the growth and metastasis of cervical cancer cells. Cancer Biomarkers, 30(3), 287-298.

Wang, W., Li, F., Gan, P., Su, D., Li, G., Dang, L., & Peng, Y. (2021). The expression of myosin-regulated light chain interacting protein (MYLIP) in lung cancer and its inhibitory effects on lung carcinomas. Translational Cancer Research, 10(5), 2389.

Zhao, L., Zhou, Y., Zhao, Y., Li, Q., Zhou, J., & Mao, Y. (2020). Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY), 12(3), 2974.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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