Mouse Anti-MYLIP (AA 127-385) Recombinant Antibody (CBFYM-2969) (CBMAB-M3164-FY)

Name: Mouse Anti-MYLIP (AA 127-385) Recombinant Antibody (CBFYM-2969)
SKU: CBMAB-M3164-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2969

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 127-385 of human MYLIP (NP_037394) produced in E.coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 127-385

More Infomation

Target

Full Name

myosin regulatory light chain interacting protein

Introduction

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.

Entrez Gene ID

29116

UniProt ID

Q8WY64

Alternative Names

Myosin Regulatory Light Chain Interacting Protein; E3 Ubiquitin Ligase-Inducible Degrader Of The Low Density Lipoprotein Receptor; RING-Type E3 Ubiquitin Transferase MYLIP; Inducible Degrader Of The LDL-Receptor; IDOL; MIR

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

Biological Process

Cholesterol homeostasis Source: Ensembl
Negative regulation of low-density lipoprotein particle clearance Source: Ensembl
Negative regulation of neuron projection development Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of protein catabolic process Source: Ensembl
Protein destabilization Source: Ensembl
Protein ubiquitination Source: UniProtKB
Regulation of low-density lipoprotein particle receptor catabolic process Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane

PTM

Autoubiquitinated.

Martins, I., Neves-Silva, D., Ascensão-Ferreira, M., Dias, A. F., Ribeiro, D., Isidro, A. F., ... & Saúde, L. (2023). Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players. International Journal of Molecular Sciences, 24(7), 6433.

Dong, Y. H., Zhou, C. J., Zhang, M. Y., Tao, J., Zhang, X. M., An, L., ... & Cang, M. (2021). MiR‐455‐5p monitors myotube morphogenesis by targeting mylip. Journal of Cellular Biochemistry, 122(3-4), 442-455.

Wang, H., Ma, L., Pan, X., Du, Z., & Chen, Y. (2021). Novel associations of SNPs MYLIP rs3757354 and ABCA1 2230806 gene with early-onset-preeclampsia: A case-control candidate genetic study. Pregnancy Hypertension, 23, 185-190.

Ni, M., Yan, Q., Xue, H., Du, Y., Zhao, S., & Zhao, Z. (2021). Identification of MYLIP gene and miRNA-802 involved in the growth and metastasis of cervical cancer cells. Cancer Biomarkers, 30(3), 287-298.

Wang, W., Li, F., Gan, P., Su, D., Li, G., Dang, L., & Peng, Y. (2021). The expression of myosin-regulated light chain interacting protein (MYLIP) in lung cancer and its inhibitory effects on lung carcinomas. Translational Cancer Research, 10(5), 2389.

Zhao, L., Zhou, Y., Zhao, Y., Li, Q., Zhou, J., & Mao, Y. (2020). Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY), 12(3), 2974.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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