Mouse Anti-PCMT1 Recombinant Antibody (2A9) (CBMAB-P1063-YC)

Name: Mouse Anti-PCMT1 Recombinant Antibody (2A9)
SKU: CBMAB-P1063-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A9

Application

WB, IHC

Immunogen

Human recombinant protein fragment corresponding to amino acids 60-285 of human PCMT1(NP_005380) produced in E. coli

Specificity

Human

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

protein-L-isoaspartate (D-aspartate) O-methyltransferase

Introduction

PCMT1 is a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure.

Entrez Gene ID

5110

UniProt ID

P22061

Alternative Names

Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase; Protein L-Isoaspartyl/D-Aspartyl Methyltransferase; L-Isoaspartyl Protein Carboxyl Methyltransferase; Protein-Beta-Aspartate Methyltransferase; EC 2.1.1.77; PIMT; Protein-L-Isoaspartate(D-Aspartate) O-Methyltransferase; EC 2.1.1;

Function

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed:3167043, PubMed:6469980).
Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

Biological Process

Protein methylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein repairManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cytoplasm, cytosol

Zhang, K., Li, J. Y., & Li, K. (2024). Silencing PCMT1 enhances the sensitivity of breast cancer cells to paclitaxel through the PI3K/Akt/STMN1 pathway. Chemical Biology & Drug Design, 103(6), e14559.

Zhong, J., Yuan, C., Liu, L., Du, Y., Hui, Y., Chen, Z., ... & Liu, X. (2023). PCMT1 regulates the migration, invasion, and apoptosis of prostate cancer through modulating the PI3K/AKT/GSK-3β pathway. Aging (Albany NY), 15(20), 11654.

Zhang, Z., Li, F., Li, Y., Li, Z., & Jia, G. (2023). In vitro anti-malignant property of PCMT1 silencing and identification of the SNHG16/miR-195/PCMT1 regulatory axis in breast cancer cells. Clinical Breast Cancer, 23(3), 302-316.

Liu, J., Liu, B., Li, Y., Mi, Z., Tan, H., & Rong, P. (2023). PCMT1 is a potential target related to tumor progression and immune infiltration in liver cancer. European Journal of Medical Research, 28(1), 289.

Guo, J., Du, X., & Li, C. (2022). PCMT1 is a potential prognostic biomarker and is correlated with immune infiltrates in breast cancer. BioMed research international, 2022(1), 4434887.

Zhang, J., Li, Y., Liu, H., Zhang, J., Wang, J., Xia, J., ... & Feng, W. (2022). Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis. Journal of Experimental & Clinical Cancer Research, 41(1), 24.

Lin, Y. W., Wu, F. C., Zhuang, Y. X., Chu, L. Y., Ding, T. Y., Qu, Q. Q., ... & Hong, C. Q. (2022). PCMT1 has Potential Prognostic Value and Promotes Cell Growth and Motility in Breast Cancer.

Yuan, C., Liu, L., Du, Y., Chen, Z., Wang, L., Liu, Z., ... & Liu, X. (2022). PCMT1 regulates the migration, invasion, and apoptosis of prostate cancer through modulating the PI3K/AKT/GSK-3β pathway.

Dong, L. M., Zhang, X. L., Mao, M. H., Li, Y. P., Zhang, X. Y., Xue, D. W., & Liu, Y. L. (2021). LINC00511/miRNA-143-3p modulates apoptosis and malignant phenotype of bladder carcinoma cells via PCMT1. Frontiers in Cell and Developmental Biology, 9, 650999.

Ozay, E. I., Shanthalingam, S., Torres, J. A., Osborne, B. A., Tew, G. N., & Minter, L. M. (2020). Protein kinase C theta modulates PCMT1 through hnRNPL to regulate FOXP3 stability in regulatory T cells. Molecular Therapy, 28(10), 2220-2236.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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