Mouse Anti-PDE5A Recombinant Antibody (1F11) (CBMAB-A6523-LY)

Name: Mouse Anti-PDE5A Recombinant Antibody (1F11)
SKU: CBMAB-A6523-LY
Rating: 5 (1 reviews)

See all PDE5A antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F11

Application

WB, ELISA

Immunogen

PDE5A (NP_001074, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

phosphodiesterase 5A, cGMP-specific

Introduction

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq]

Entrez Gene ID

8654

UniProt ID

O76074

Alternative Names

CGB-PDE; CN5A; PDE5; PDE5A1

Function

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP (PubMed:9714779, PubMed:15489334).
Specifically regulates nitric-oxide-generated cGMP (PubMed:15489334).

Biological Process

cGMP catabolic processIEA:UniProtKB-UniPathway
Negative regulation of cardiac muscle contractionIEA:Ensembl
Negative regulation of T cell proliferationIEA:Ensembl
Positive regulation of cardiac muscle hypertrophyIEA:Ensembl
Positive regulation of MAP kinase activityIEA:Ensembl
Positive regulation of oocyte developmentIEA:Ensembl
Regulation of nitric oxide mediated signal transductionIEA:Ensembl
Relaxation of cardiac muscleIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytosol

PTM

Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity).
Phosphorylation by PRKG1 leads to its activation.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PDE5A Recombinant Antibody (1F11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PDE5A Recombinant Antibody (CBYY-C0320) (CAT#: CBMAB-C1078-YY)

Mouse Anti-PDE5A Recombinant Antibody (D-3) (CAT#: CBMAB-P1223-YC)

Mouse Anti-PDE5A Recombinant Antibody (9H5) (CAT#: CBMAB-P1222-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry