Mouse Anti-PELI1 Recombinant Antibody (14F10) (CBMAB-P1430-YC)

Name: Mouse Anti-PELI1 Recombinant Antibody (14F10)
SKU: CBMAB-P1430-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

14F10

Application

IHC, WB

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-245 of human PELI1(NP_065702) produced in E. coli

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Pellino E3 Ubiquitin Protein Ligase 1

Introduction

PELI1 has broad expression in bone marrow (RPKM 57.0), esophagus (RPKM 14.7) and 19 other tissues.

Entrez Gene ID

57162

UniProt ID

Q96FA3

Alternative Names

Pellino E3 Ubiquitin Protein Ligase 1; RING-Type E3 Ubiquitin Transferase Pellino Homolog 1; Pellino-Related Intracellular-Signaling Molecule; Pellino-1; E3 Ubiquitin-Protein Ligase Pellino Homolog 1; Pellino (Drosophila) Homolog 1;

Function

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297).
Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609).
Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609).
Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609).

Biological Process

Negative regulation of necroptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of NF-kappaB transcription factor activityIEA:Ensembl
Negative regulation of T cell proliferationIEA:Ensembl
Positive regulation of B cell proliferationIEA:Ensembl
Positive regulation of cytokine productionIEA:Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signalingTAS:Reactome
Positive regulation of protein ubiquitinationIEA:Ensembl
Positive regulation of toll-like receptor 3 signaling pathwayIEA:Ensembl
Positive regulation of toll-like receptor 4 signaling pathwayIEA:Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein K63-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein polyubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of necroptotic processTAS:Reactome
Regulation of Toll signaling pathwayIEA:InterPro
Response to dsRNAIEA:Ensembl
Response to lipopolysaccharideManual Assertion Based On ExperimentIDA:ARUK-UCL

Cellular Location

cytosol
nucleus

PTM

Phosphorylation by IRAK1 and IRAK4 enhances its E3 ligase activity.
Sumoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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