Human Recombinant PELI1 protein, GST Tag (V2LY-0526-LY6102)

Name: Human Recombinant PELI1 protein, GST Tag
SKU: V2LY-0526-LY6102
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant PELI1 protein, GST Tag consist of Amino Acid: 1-418 and predicts a molecular mass of 74.1 kDa.

Molecule Mass

74.1 kDa

Sequence

Amino Acid: 1-418

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Pellino E3 Ubiquitin Protein Ligase 1

Function

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297).
Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609).
Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609).
Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609).

Biological Process

Negative regulation of necroptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of NF-kappaB transcription factor activityIEA:Ensembl
Negative regulation of T cell proliferationIEA:Ensembl
Positive regulation of B cell proliferationIEA:Ensembl
Positive regulation of cytokine productionIEA:Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signalingTAS:Reactome
Positive regulation of protein ubiquitinationIEA:Ensembl
Positive regulation of toll-like receptor 3 signaling pathwayIEA:Ensembl
Positive regulation of toll-like receptor 4 signaling pathwayIEA:Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein K63-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein polyubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of necroptotic processTAS:Reactome
Regulation of Toll signaling pathwayIEA:InterPro
Response to dsRNAIEA:Ensembl
Response to lipopolysaccharideManual Assertion Based On ExperimentIDA:ARUK-UCL

Cellular Location

cytosol
nucleus

PTM

Phosphorylation by IRAK1 and IRAK4 enhances its E3 ligase activity.
Sumoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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